Detailed information of TA system TA183412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1919248..1919852
• Replicon: chromosome
• Accession: NZ_CP065824
• Organism: Pseudomonas tolaasii strain Pt11

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: I7845_RS08715
• Protein ID: WP_218820239.1
• Coordinates: 1919248..1919436 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2T4FRT1
• Locus tag: I7845_RS08720
• Protein ID: WP_016978509.1
• Coordinates: 1919448..1919852 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I7845_RS08690 (I7845_08690)	1915021..1915830	+	810	WP_165448264.1	phosphonate C-P lyase system protein PhnK	-
I7845_RS08695 (I7845_08695)	1915827..1916549	+	723	WP_080519880.1	phosphonate C-P lyase system protein PhnL	-
I7845_RS08700 (I7845_08700)	1916539..1917684	+	1146	WP_177038239.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
I7845_RS08705 (I7845_08705)	1917684..1918256	+	573	WP_177038238.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
I7845_RS08710 (I7845_08710)	1918247..1918996	+	750	WP_177006963.1	phosphonate metabolism protein PhnP	-
I7845_RS08715 (I7845_08715)	1919248..1919436	+	189	WP_218820239.1	type II toxin-antitoxin system HicA family toxin	Toxin
I7845_RS08720 (I7845_08720)	1919448..1919852	+	405	WP_016978509.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I7845_RS08725 (I7845_08725)	1919923..1921119	-	1197	WP_017255988.1	MFS transporter	-
I7845_RS08730 (I7845_08730)	1921298..1921912	+	615	WP_016972132.1	DUF5666 domain-containing protein	-
I7845_RS08735 (I7845_08735)	1922029..1923033	-	1005	WP_016972133.1	sulfate ABC transporter substrate-binding protein	-
I7845_RS08740 (I7845_08740)	1923139..1923963	-	825	WP_177006964.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7193.38 Da
• Isoelectric Point: 10.8676

>T183412 WP_218820239.1 NZ_CP065824:1919248-1919436 [Pseudomonas tolaasii]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYSPYTIPVPHPKKDLPLGTVRSIRKRAGLFEF

Nucleotide

• Length: 189 bp

>T183412 NZ_CP086980:23994-24389 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14701.68 Da
• Isoelectric Point: 4.6000

>AT183412 WP_016978509.1 NZ_CP065824:1919448-1919852 [Pseudomonas tolaasii]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGPIPMPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT183412 NZ_CP086980:23777-24004 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4FRT1