Detailed information of TA system TA183404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 367693..368232
• Replicon: chromosome
• Accession: NZ_CP065795
• Organism: Mammaliicoccus sciuri strain GDH8C110P

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I7830_RS01955
• Protein ID: WP_103361834.1
• Coordinates: 367693..368064 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A657XHG2
• Locus tag: I7830_RS01960
• Protein ID: WP_025904510.1
• Coordinates: 368065..368232 (-)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I7830_RS01935	364938..365708	-	771	WP_025904515.1	RNA polymerase sigma factor SigB	-
I7830_RS01940	365683..366159	-	477	WP_025904514.1	anti-sigma B factor RsbW	-
I7830_RS01945	366164..366487	-	324	WP_025904513.1	anti-sigma factor antagonist	-
I7830_RS01950	366592..367596	-	1005	WP_103361835.1	PP2C family protein-serine/threonine phosphatase	-
I7830_RS01955	367693..368064	-	372	WP_103361834.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I7830_RS01960	368065..368232	-	168	WP_025904510.1	hypothetical protein	Antitoxin
I7830_RS01965	368317..369465	-	1149	WP_103361833.1	alanine racemase	-
I7830_RS01970	369513..369875	-	363	WP_103361832.1	holo-ACP synthase	-
I7830_RS01975	369906..370370	-	465	WP_103361831.1	PH domain-containing protein	-
I7830_RS01980	370360..371880	-	1521	WP_198052263.1	PH domain-containing protein	-
I7830_RS01985	371870..372349	-	480	WP_103361829.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13671.80 Da
• Isoelectric Point: 9.9193

>T183404 WP_103361834.1 NZ_CP065795:c368064-367693 [Mammaliicoccus sciuri]
MRRGDVYLADLSPVTGSEQGGTRPVVIIQNDTGNRYSPTVIVAAITGKINKAKIPTHVEIEAAKYKLDRDSVILLEQIRT
IDKKRLKEKLTYLSDTKMKEVDTAIAISLNLTLQQKFDTLGNT

Nucleotide

• Length: 372 bp

>T183404 NZ_CP086973:92528-92779 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6136.65 Da
• Isoelectric Point: 4.0121

>AT183404 WP_025904510.1 NZ_CP065795:c368232-368065 [Mammaliicoccus sciuri]
MTSLTQNRTQSLEQTLKEGYRSMADLNLSLASEAYYCECEACDSNESHIASQQGE

Nucleotide

• Length: 168 bp

>AT183404 NZ_CP086973:92776-93042 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Structures

Antitoxin

• AlphaFold DB: A0A657XHG2