Detailed information of TA system TA183400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 631341..632449
• Replicon: chromosome
• Accession: NZ_CP065792
• Organism: Mammaliicoccus sciuri strain GDK8D6P

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: I7827_RS03230
• Protein ID: WP_000233000.1
• Coordinates: 631341..632210 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: I7827_RS03235
• Protein ID: WP_000205227.1
• Coordinates: 632225..632449 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I7827_RS03205	627764..627802	-	39	WP_193221770.1	hypothetical protein	-
I7827_RS03210	628592..628795	-	204	WP_017370349.1	conjugal transfer protein	-
I7827_RS03215	628879..629559	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
I7827_RS03220	629730..630593	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
I7827_RS03225	630626..631360	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
I7827_RS03230	631341..632210	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
I7827_RS03235	632225..632449	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
I7827_RS03240	632592..634136	-	1545	WP_002390960.1	recombinase family protein	-
I7827_RS03245	634156..634572	-	417	WP_000323438.1	recombinase	-
I7827_RS03250	634573..634869	-	297	WP_002360685.1	hypothetical protein	-
I7827_RS03255	635417..636580	-	1164	WP_025905227.1	chromate efflux transporter	-
I7827_RS03260	636591..636893	-	303	WP_025905228.1	helix-turn-helix transcriptional regulator	-
I7827_RS03265	637019..637420	-	402	WP_025905229.1	arsenate reductase (thioredoxin)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(S) / ant(6)-Ia	-	622718..634572	11854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T183400 WP_000233000.1 NZ_CP065792:c632210-631341 [Mammaliicoccus sciuri]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T183400 NZ_CP086970:c23602-23351 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT183400 WP_000205227.1 NZ_CP065792:c632449-632225 [Mammaliicoccus sciuri]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT183400 NZ_CP086970:c23354-23088 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Structures

Toxin

• AlphaFold DB: R3JIN1