Detailed information of TA system TA183399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1315288..1316205
• Replicon: chromosome
• Accession: NZ_CP065791
• Organism: Bacillus velezensis strain LBUM279

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: I8N73_RS06740
• Protein ID: WP_015239694.1
• Coordinates: 1315459..1316205 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: I8N73_RS06735
• Protein ID: WP_003154807.1
• Coordinates: 1315288..1315458 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N73_RS06700	1310500..1312089	+	1590	WP_015239691.1	hypothetical protein	-
I8N73_RS06705	1312102..1312527	+	426	WP_051483351.1	hypothetical protein	-
I8N73_RS06710	1312532..1312729	+	198	WP_012117366.1	XkdX family protein	-
I8N73_RS06715	1312786..1313547	+	762	WP_043021265.1	hypothetical protein	-
I8N73_RS06720	1313599..1313862	+	264	WP_003154815.1	hemolysin XhlA family protein	-
I8N73_RS06725	1313876..1314139	+	264	WP_003154813.1	phage holin	-
I8N73_RS06730	1314153..1315031	+	879	WP_199022485.1	N-acetylmuramoyl-L-alanine amidase	-
I8N73_RS06735	1315288..1315458	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
I8N73_RS06740	1315459..1316205	-	747	WP_015239694.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
I8N73_RS06745	1316313..1317311	-	999	WP_007407255.1	inorganic phosphate transporter	-
I8N73_RS06750	1317324..1317941	-	618	WP_003154804.1	DUF47 domain-containing protein	-
I8N73_RS06755	1318228..1319544	-	1317	WP_007610842.1	amino acid permease	-
I8N73_RS06760	1319867..1320817	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29135.61 Da
• Isoelectric Point: 4.7003

>T183399 WP_015239694.1 NZ_CP065791:c1316205-1315459 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T183399 NZ_CP086970:c23354-23088 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT183399 WP_003154807.1 NZ_CP065791:c1315458-1315288 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT183399 NZ_CP086970:c23602-23351 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14