Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 538129..538766 Replicon chromosome
Accession NZ_CP065791
Organism Bacillus velezensis strain LBUM279

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag I8N73_RS02600 Protein ID WP_003156187.1
Coordinates 538416..538766 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag I8N73_RS02595 Protein ID WP_003156188.1
Coordinates 538129..538410 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N73_RS02575 534494..535093 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
I8N73_RS02580 535186..535551 + 366 WP_007410229.1 holo-ACP synthase -
I8N73_RS02585 535716..536723 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
I8N73_RS02590 536840..538009 + 1170 WP_039252648.1 alanine racemase -
I8N73_RS02595 538129..538410 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
I8N73_RS02600 538416..538766 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I8N73_RS02605 538884..539705 + 822 WP_003156182.1 STAS domain-containing protein -
I8N73_RS02610 539710..540075 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
I8N73_RS02615 540078..540479 + 402 WP_003156178.1 anti-sigma regulatory factor -
I8N73_RS02620 540491..541498 + 1008 WP_039252645.1 PP2C family protein-serine/threonine phosphatase -
I8N73_RS02625 541562..541891 + 330 WP_031378636.1 anti-sigma factor antagonist -
I8N73_RS02630 541888..542370 + 483 WP_007609591.1 anti-sigma B factor RsbW -
I8N73_RS02635 542336..543124 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
I8N73_RS02640 543124..543726 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T183398 WP_003156187.1 NZ_CP065791:538416-538766 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T183398 NZ_CP086968:39103-39378 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT183398 WP_003156188.1 NZ_CP065791:538129-538410 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT183398 NZ_CP086968:38817-39110 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References