Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1266030..1266947 Replicon chromosome
Accession NZ_CP065790
Organism Bacillus velezensis strain LBUM1082

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag I8N72_RS06655 Protein ID WP_003154806.1
Coordinates 1266201..1266947 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag I8N72_RS06650 Protein ID WP_003154807.1
Coordinates 1266030..1266200 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N72_RS06615 1261261..1262883 + 1623 WP_014304855.1 pyocin knob domain-containing protein -
I8N72_RS06620 1262896..1263267 + 372 WP_014304856.1 hypothetical protein -
I8N72_RS06625 1263273..1263470 + 198 WP_003154819.1 XkdX family protein -
I8N72_RS06630 1263527..1264288 + 762 WP_014304857.1 hypothetical protein -
I8N72_RS06635 1264340..1264603 + 264 WP_003154815.1 hemolysin XhlA family protein -
I8N72_RS06640 1264617..1264880 + 264 WP_003154813.1 phage holin -
I8N72_RS06645 1264894..1265772 + 879 WP_014304858.1 N-acetylmuramoyl-L-alanine amidase -
I8N72_RS06650 1266030..1266200 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
I8N72_RS06655 1266201..1266947 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
I8N72_RS06660 1267052..1268050 - 999 WP_003154805.1 inorganic phosphate transporter -
I8N72_RS06665 1268063..1268680 - 618 WP_003154804.1 DUF47 domain-containing protein -
I8N72_RS06670 1268966..1270282 - 1317 WP_003154801.1 amino acid permease -
I8N72_RS06675 1270606..1271556 + 951 WP_015388352.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T183396 WP_003154806.1 NZ_CP065790:c1266947-1266201 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T183396 NZ_CP086966:103767-104018 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT183396 WP_003154807.1 NZ_CP065790:c1266200-1266030 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT183396 NZ_CP086966:104015-104281 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References