Detailed information of TA system TA183395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 472647..473284
• Replicon: chromosome
• Accession: NZ_CP065790
• Organism: Bacillus velezensis strain LBUM1082

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: I8N72_RS02400
• Protein ID: WP_003156187.1
• Coordinates: 472934..473284 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: I8N72_RS02395
• Protein ID: WP_003156188.1
• Coordinates: 472647..472928 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N72_RS02375	469012..469611	-	600	WP_015388676.1	rhomboid family intramembrane serine protease	-
I8N72_RS02380	469704..470069	+	366	WP_014304402.1	holo-ACP synthase	-
I8N72_RS02385	470234..471241	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
I8N72_RS02390	471358..472527	+	1170	WP_015388675.1	alanine racemase	-
I8N72_RS02395	472647..472928	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
I8N72_RS02400	472934..473284	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
I8N72_RS02405	473402..474223	+	822	WP_003156182.1	STAS domain-containing protein	-
I8N72_RS02410	474228..474593	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
I8N72_RS02415	474596..474997	+	402	WP_003156178.1	anti-sigma regulatory factor	-
I8N72_RS02420	475009..476016	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
I8N72_RS02425	476080..476409	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
I8N72_RS02430	476406..476888	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
I8N72_RS02435	476854..477642	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
I8N72_RS02440	477642..478244	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T183395 WP_003156187.1 NZ_CP065790:472934-473284 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T183395 NZ_CP086965:c91391-90996 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT183395 WP_003156188.1 NZ_CP065790:472647-472928 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT183395 NZ_CP086965:c91608-91381 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5