Detailed information of TA system TA183373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1348045..1348961
• Replicon: chromosome
• Accession: NZ_CP065789
• Organism: Bacillus subtilis strain LBUM979

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: I8N71_RS07080
• Protein ID: WP_003244695.1
• Coordinates: 1348215..1348961 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: I8N71_RS07075
• Protein ID: WP_003232646.1
• Coordinates: 1348045..1348215 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N71_RS07040	1344908..1345237	+	330	WP_003232660.1	phage-like element PBSX protein XkdW	-
I8N71_RS07045	1345234..1345398	+	165	WP_003232658.1	XkdX family protein	-
I8N71_RS07050	1345442..1346281	+	840	WP_003245597.1	phage-like element PBSX protein XepA	-
I8N71_RS07055	1346334..1346603	+	270	WP_003232655.1	hemolysin XhlA family protein	-
I8N71_RS07060	1346616..1346879	+	264	WP_003232653.1	phage holin	-
I8N71_RS07065	1346892..1347785	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
I8N71_RS07070	1347822..1347959	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
I8N71_RS07075	1348045..1348215	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
I8N71_RS07080	1348215..1348961	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
I8N71_RS07085	1349071..1350072	-	1002	WP_003232642.1	inorganic phosphate transporter	-
I8N71_RS07090	1350085..1350702	-	618	WP_003218470.1	DUF47 domain-containing protein	-
I8N71_RS07095	1350978..1352294	-	1317	WP_003244819.1	serine/threonine exchanger	-
I8N71_RS07100	1352683..1353633	+	951	WP_003245086.1	ring-cleaving dioxygenase	-
I8N71_RS07105	1353734..1353880	+	147	WP_003244977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T183373 WP_003244695.1 NZ_CP065789:c1348961-1348215 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T183373 NZ_CP086941:86406-86672 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT183373 WP_003232646.1 NZ_CP065789:c1348215-1348045 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT183373 NZ_CP086941:86158-86409 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8