Detailed information of TA system TA183371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 35367..35901
• Replicon: plasmid p18-243_2
• Accession: NZ_CP065786
• Organism: Enterococcus faecalis strain 18-243

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: I8F59_RS15155
• Protein ID: WP_002360769.1
• Coordinates: 35626..35901 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: I8F59_RS15150
• Protein ID: WP_002369771.1
• Coordinates: 35367..35633 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8F59_RS15120 (I8F59_15120)	31471..32256	+	786	WP_002385543.1	replication-relaxation family protein	-
I8F59_RS15125 (I8F59_15125)	32262..32750	+	489	WP_002385542.1	hypothetical protein	-
I8F59_RS15130 (I8F59_15130)	33342..33608	+	267	WP_025187532.1	hypothetical protein	-
I8F59_RS15135 (I8F59_15135)	33641..34135	+	495	WP_002385540.1	DnaJ domain-containing protein	-
I8F59_RS15140 (I8F59_15140)	34232..34648	+	417	WP_010830229.1	single-stranded DNA-binding protein	-
I8F59_RS15145 (I8F59_15145)	34672..35256	+	585	WP_010830230.1	thermonuclease family protein	-
I8F59_RS15150 (I8F59_15150)	35367..35633	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I8F59_RS15155 (I8F59_15155)	35626..35901	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
I8F59_RS15160 (I8F59_15160)	36704..36889	+	186	WP_002365983.1	hypothetical protein	-
I8F59_RS15165 (I8F59_15165)	36874..37065	+	192	WP_002385537.1	DpnD/PcfM family protein	-
I8F59_RS15170 (I8F59_15170)	37285..38064	+	780	WP_214333474.1	Cas9 inhibitor AcrIIA9 family protein	-
I8F59_RS15175 (I8F59_15175)	38064..39407	+	1344	WP_048942587.1	PcfJ domain-containing protein	-
I8F59_RS15180 (I8F59_15180)	40098..40826	+	729	WP_025187535.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	poxtA	prgB/asc10	1..51905	51905

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T183371 WP_002360769.1 NZ_CP065786:35626-35901 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T183371 NZ_CP086939:10971-11237 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT183371 WP_002369771.1 NZ_CP065786:35367-35633 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT183371 NZ_CP086939:10723-10974 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7