Detailed information of TA system TA183370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 14303..14799
• Replicon: plasmid p18-243_1
• Accession: NZ_CP065785
• Organism: Enterococcus faecalis strain 18-243

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3GZA4
• Locus tag: I8F59_RS14780
• Protein ID: WP_002358470.1
• Coordinates: 14530..14799 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R3JPM7
• Locus tag: I8F59_RS14775
• Protein ID: WP_002413218.1
• Coordinates: 14303..14530 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8F59_RS14755 (I8F59_14755)	10034..11401	+	1368	WP_025188086.1	bacteriocin secretion accessory protein	-
I8F59_RS14760 (I8F59_14760)	12192..12455	+	264	WP_002358468.1	hypothetical protein	-
I8F59_RS14765 (I8F59_14765)	12448..13005	+	558	WP_002358242.1	hypothetical protein	-
I8F59_RS14770 (I8F59_14770)	13008..14243	+	1236	WP_010717251.1	MobP2 family relaxase	-
I8F59_RS14775 (I8F59_14775)	14303..14530	+	228	WP_002413218.1	DUF6290 family protein	Antitoxin
I8F59_RS14780 (I8F59_14780)	14530..14799	+	270	WP_002358470.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I8F59_RS14785 (I8F59_14785)	14866..16446	+	1581	WP_002404189.1	DUF3991 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..53948	53948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 10.5395

>T183370 WP_002358470.1 NZ_CP065785:14530-14799 [Enterococcus faecalis]
MSYRLEFTPDVKKQLKKMDKYQATLLTRWLYRNIDGTANPREHGKALSANRAGQWRYRIGNYRVIVEIEDERLVVTAIQV
GHRRNVYDK

Nucleotide

• Length: 270 bp

>T183370 NZ_CP086939:10723-10974 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8785.90 Da
• Isoelectric Point: 4.0179

>AT183370 WP_002413218.1 NZ_CP065785:14303-14530 [Enterococcus faecalis]
MAMITVRVSDEEKEWLSYMAEFFGVTLSDLLKSYSMEQLEDEYDKQTAEIAHKRWIEQGKETISMDEILNEFGGL

Nucleotide

• Length: 228 bp

>AT183370 NZ_CP086939:10971-11237 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Structures

Toxin

• AlphaFold DB: R3GZA4

Antitoxin

• AlphaFold DB: R3JPM7