Detailed information of TA system TA183340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 77320..78428
• Replicon: plasmid p16-021_1
• Accession: NZ_CP065778
• Organism: Enterococcus faecium strain 16-021

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: I8F57_RS13570
• Protein ID: WP_000233000.1
• Coordinates: 77320..78189 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: I8F57_RS13575
• Protein ID: WP_000205227.1
• Coordinates: 78204..78428 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8F57_RS13545 (I8F57_13540)	72429..73439	-	1011	Protein_80	DNA topoisomerase	-
I8F57_RS13550 (I8F57_13545)	73591..74910	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
I8F57_RS13555 (I8F57_13550)	75138..75665	-	528	WP_002294507.1	phosphoribosyltransferase family protein	-
I8F57_RS13560 (I8F57_13555)	75709..76572	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
I8F57_RS13565 (I8F57_13560)	76605..77339	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
I8F57_RS13570 (I8F57_13565)	77320..78189	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
I8F57_RS13575 (I8F57_13570)	78204..78428	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
I8F57_RS13580 (I8F57_13575)	78866..80148	+	1283	Protein_87	ISL3 family transposase	-
I8F57_RS13585 (I8F57_13580)	80457..81260	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
I8F57_RS13590 (I8F57_13585)	81314..82798	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T183340 WP_000233000.1 NZ_CP065778:c78189-77320 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T183340 NZ_CP086909:986053-986316 [Pseudosulfitobacter pseudonitzschiae]
ATGAAGTTCGAGTATGACCCTGACAAGAGTGCCGGGAACAAGGCCAAGCACGGCATCGACTTCGATGAAGCGCAAGCGCT
TTGGGAAGACCCGTGGCTGTTGGAGGCTCCCGCTCGAACAGATGACGAGCCGCGCTTTTTTGCAATAGGCATGATCAAGG
AACGGCATTGGTCAGCAGTTTGCACCTATCGCGGTGATATTATTCGGATCATCTCGGTGCGCCGCGCCAGAAAACAGGAG
ATTGAGCACTATGAAAGCACGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT183340 WP_000205227.1 NZ_CP065778:c78428-78204 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT183340 NZ_CP086909:986303-986518 [Pseudosulfitobacter pseudonitzschiae]
ATGAAAGCACGTGAGTTCGATCAAAAATTTGACACAGGTGAGAACGTCAGCGATCTTGTGGACTGGTCCAAAGCTAGGCG
GACGAATACCCAGACGAAGCGAGTCAATGTGGATTTTCCGTCGTGGGTCGTTTCTGGTTTGGATCAGAAGGCAAAGCAGC
TCGGCGTTACTCGTCAGTCGCTCATCAAACTCTGGATCGCCGAGCGGCTCCAATGA

Structures

Toxin

• AlphaFold DB: R3JIN1