Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 578726..579297 Replicon chromosome
Accession NZ_CP065777
Organism Enterococcus faecium strain 16-021

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag I8F57_RS02800 Protein ID WP_002286801.1
Coordinates 578956..579297 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag I8F57_RS02795 Protein ID WP_002323011.1
Coordinates 578726..578956 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8F57_RS02765 (I8F57_02765) 573859..575190 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
I8F57_RS02770 (I8F57_02770) 575212..575838 + 627 WP_002286816.1 cysteine hydrolase -
I8F57_RS02775 (I8F57_02775) 576021..576602 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
I8F57_RS14215 577334..577896 + 563 Protein_551 SOS response-associated peptidase -
I8F57_RS02790 (I8F57_02790) 578101..578439 - 339 WP_002286804.1 hypothetical protein -
I8F57_RS02795 (I8F57_02795) 578726..578956 + 231 WP_002323011.1 hypothetical protein Antitoxin
I8F57_RS02800 (I8F57_02800) 578956..579297 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I8F57_RS02805 (I8F57_02805) 580147..580332 + 186 WP_002304207.1 hypothetical protein -
I8F57_RS02810 (I8F57_02810) 580837..581031 + 195 WP_002297028.1 hypothetical protein -
I8F57_RS02815 (I8F57_02815) 581222..581471 + 250 Protein_557 transposase -
I8F57_RS02820 (I8F57_02820) 581715..582545 - 831 WP_002286789.1 manganese catalase family protein -
I8F57_RS02825 (I8F57_02825) 582753..584087 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T183339 WP_002286801.1 NZ_CP065777:578956-579297 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T183339 NZ_CP086908:c18438-18187 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT183339 WP_002323011.1 NZ_CP065777:578726-578956 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT183339 NZ_CP086908:c18190-17924 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References