Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Xre-MNT/HTH_26(antitoxin) |
| Location | 135883..136991 | Replicon | plasmid p16-164_1 |
| Accession | NZ_CP065774 | ||
| Organism | Enterococcus faecium strain 16-164 | ||
Toxin (Protein)
| Gene name | MNTss | Uniprot ID | R3JIN1 |
| Locus tag | I8F58_RS12885 | Protein ID | WP_000233000.1 |
| Coordinates | 135883..136752 (-) | Length | 290 a.a. |
Antitoxin (Protein)
| Gene name | Xress | Uniprot ID | - |
| Locus tag | I8F58_RS12890 | Protein ID | WP_000205227.1 |
| Coordinates | 136767..136991 (-) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I8F58_RS12850 (I8F58_12865) | 131318..132055 | - | 738 | WP_002321849.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
| I8F58_RS12855 (I8F58_12870) | 132180..132259 | - | 80 | Protein_146 | 23S rRNA methyltransferase attenuation leader peptide | - |
| I8F58_RS12865 (I8F58_12880) | 132801..133595 | - | 795 | WP_001096887.1 | aminoglycoside O-phosphotransferase APH(3')-IIIa | - |
| I8F58_RS12870 (I8F58_12885) | 133688..134230 | - | 543 | WP_000627290.1 | streptothricin N-acetyltransferase Sat4 | - |
| I8F58_RS12875 (I8F58_12890) | 134227..135135 | - | 909 | WP_001255866.1 | aminoglycoside nucleotidyltransferase ANT(6)-Ia | - |
| I8F58_RS12880 (I8F58_12895) | 135168..135902 | - | 735 | WP_000662263.1 | class I SAM-dependent methyltransferase | - |
| I8F58_RS12885 (I8F58_12900) | 135883..136752 | - | 870 | WP_000233000.1 | nucleotidyltransferase domain-containing protein | Toxin |
| I8F58_RS12890 (I8F58_12905) | 136767..136991 | - | 225 | WP_000205227.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| I8F58_RS13920 | 137134..137363 | - | 230 | Protein_153 | hypothetical protein | - |
| I8F58_RS12895 (I8F58_12910) | 137429..137671 | + | 243 | WP_214308132.1 | hypothetical protein | - |
| I8F58_RS12900 (I8F58_12915) | 137753..138712 | + | 960 | WP_002301399.1 | IS30 family transposase | - |
| I8F58_RS12905 (I8F58_12920) | 138716..139776 | + | 1061 | Protein_156 | ISL3 family transposase | - |
| I8F58_RS12915 (I8F58_12930) | 140085..140888 | - | 804 | WP_002294514.1 | lincosamide nucleotidyltransferase Lnu(B) | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 290 a.a. Molecular weight: 32926.57 Da Isoelectric Point: 4.8781
>T183337 WP_000233000.1 NZ_CP065774:c136752-135883 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
Download Length: 870 bp
>T183337 NZ_CP086907:39103-39378 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA
Antitoxin
Download Length: 75 a.a. Molecular weight: 8365.68 Da Isoelectric Point: 4.5510
>AT183337 WP_000205227.1 NZ_CP065774:c136991-136767 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
Download Length: 225 bp
>AT183337 NZ_CP086907:38817-39110 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|