Detailed information of TA system TA183336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 461831..462402
• Replicon: chromosome
• Accession: NZ_CP065773
• Organism: Enterococcus faecium strain 16-164

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I8F58_RS02180
• Protein ID: WP_002327666.1
• Coordinates: 462061..462402 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: I8F58_RS02175
• Protein ID: WP_002323011.1
• Coordinates: 461831..462061 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8F58_RS02150 (I8F58_02150)	457232..457858	+	627	WP_002286816.1	cysteine hydrolase	-
I8F58_RS02155 (I8F58_02155)	458041..458622	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
I8F58_RS02160 (I8F58_02160)	459354..459929	+	576	WP_002327664.1	SOS response-associated peptidase family protein	-
I8F58_RS02165 (I8F58_02165)	460130..460468	-	339	WP_002327665.1	hypothetical protein	-
I8F58_RS02170 (I8F58_02170)	460712..461671	+	960	WP_000222577.1	IS30-like element IS1252 family transposase	-
I8F58_RS02175 (I8F58_02175)	461831..462061	+	231	WP_002323011.1	hypothetical protein	Antitoxin
I8F58_RS02180 (I8F58_02180)	462061..462402	+	342	WP_002327666.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I8F58_RS02190 (I8F58_02190)	464621..464806	+	186	WP_002304207.1	hypothetical protein	-
I8F58_RS02195 (I8F58_02195)	465283..466445	+	1163	WP_086956687.1	IS3 family transposase	-
I8F58_RS02200 (I8F58_02200)	466680..466874	+	195	WP_002295146.1	hypothetical protein	-
I8F58_RS02205 (I8F58_02205)	467065..467314	+	250	Protein_438	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	458041..466445	8404
	-	inside	IScluster/Tn	-	-	460712..466445	5733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13264.67 Da
• Isoelectric Point: 10.1461

>T183336 WP_002327666.1 NZ_CP065773:462061-462402 [Enterococcus faecium]
VSGERIYIPKKGDIVWIYFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T183336 NZ_CP086905:c178490-178095 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT183336 WP_002323011.1 NZ_CP065773:461831-462061 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT183336 NZ_CP086905:c178707-178480 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9