Detailed information of TA system TA183335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 181430..181959
• Replicon: chromosome
• Accession: NZ_CP065773
• Organism: Enterococcus faecium strain 16-164

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C9B8W9
• Locus tag: I8F58_RS00905
• Protein ID: WP_002294198.1
• Coordinates: 181693..181959 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: I8F58_RS00900
• Protein ID: WP_105459660.1
• Coordinates: 181430..181693 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8F58_RS00890 (I8F58_00890)	178805..180628	+	1824	WP_002294202.1	APC family permease	-
I8F58_RS00895 (I8F58_00895)	180770..180955	-	186	WP_002294201.1	hypothetical protein	-
I8F58_RS13680	180962..181090	+	129	WP_002294200.1	hypothetical protein	-
I8F58_RS00900 (I8F58_00900)	181430..181693	+	264	WP_105459660.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I8F58_RS00905 (I8F58_00905)	181693..181959	+	267	WP_002294198.1	Txe/YoeB family addiction module toxin	Toxin
I8F58_RS00910 (I8F58_00910)	182160..182648	+	489	WP_002294196.1	mannosyl-glycoprotein endo-beta-N-acetylglucosamidase	-
I8F58_RS00915 (I8F58_00915)	182711..182878	+	168	WP_002294195.1	hypothetical protein	-
I8F58_RS00920 (I8F58_00920)	183190..183843	-	654	WP_002317081.1	ABC transporter permease	-
I8F58_RS00925 (I8F58_00925)	183858..184775	-	918	WP_002294193.1	osmoprotectant ABC transporter substrate-binding protein	-
I8F58_RS00930 (I8F58_00930)	184772..185413	-	642	WP_002293405.1	ABC transporter permease	-
I8F58_RS00935 (I8F58_00935)	185417..186592	-	1176	WP_002294192.1	betaine/proline/choline family ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	186784..187743	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (22-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10646.11 Da
• Isoelectric Point: 9.9931

>T183335 WP_002294198.1 NZ_CP065773:181693-181959 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Nucleotide

• Length: 267 bp

>T183335 NZ_CP086903:104016-104282 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10044.08 Da
• Isoelectric Point: 4.1207

>AT183335 WP_105459660.1 NZ_CP065773:181430-181693 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRVIQETLFLEQTGTLDKVRDREKDDSGFTNID
DIDWEAL

Nucleotide

• Length: 264 bp

>AT183335 NZ_CP086903:103768-104019 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A829FMA0