Detailed information of TA system TA183334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 74294..75402
• Replicon: plasmid p17-318_1
• Accession: NZ_CP065771
• Organism: Enterococcus faecium strain 17-318

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: I8F52_RS13290
• Protein ID: WP_071661597.1
• Coordinates: 74294..75163 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: I8F52_RS13295
• Protein ID: WP_000205227.1
• Coordinates: 75178..75402 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8F52_RS13260 (I8F52_13250)	70253..70912	-	660	Protein_82	aminoglycoside phosphotransferase family protein	-
I8F52_RS13265 (I8F52_13255)	70964..72136	-	1173	WP_104807992.1	IS256-like element IS1542 family transposase	-
I8F52_RS13270 (I8F52_13260)	72236..73024	-	789	Protein_84	aminoglycoside N-acetyltransferase AAC(6')-Ie	-
I8F52_RS13275 (I8F52_13265)	73025..73417	-	393	WP_000393259.1	GNAT family N-acetyltransferase	-
I8F52_RS13280 (I8F52_13270)	73436..73546	-	111	Protein_86	aminoglycoside 6-adenylyltransferase	-
I8F52_RS13285 (I8F52_13275)	73579..74313	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
I8F52_RS13290 (I8F52_13280)	74294..75163	-	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
I8F52_RS13295 (I8F52_13285)	75178..75402	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
I8F52_RS13300 (I8F52_13290)	75820..77121	+	1302	Protein_90	ISL3 family transposase	-
I8F52_RS13305 (I8F52_13295)	77430..78233	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
I8F52_RS13310 (I8F52_13300)	78287..79771	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T183334 WP_071661597.1 NZ_CP065771:c75163-74294 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T183334 NZ_CP086903:103768-104019 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT183334 WP_000205227.1 NZ_CP065771:c75402-75178 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT183334 NZ_CP086903:104016-104282 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6