Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 468401..468972 Replicon chromosome
Accession NZ_CP065767
Organism Enterococcus faecium strain 17-508

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag I8F56_RS02225 Protein ID WP_002302307.1
Coordinates 468631..468972 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag I8F56_RS02220 Protein ID WP_002323011.1
Coordinates 468401..468631 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8F56_RS02195 (I8F56_02195) 463890..465218 + 1329 WP_044383738.1 FAD-dependent oxidoreductase -
I8F56_RS02200 (I8F56_02200) 465240..465866 + 627 WP_002286816.1 cysteine hydrolase -
I8F56_RS02205 (I8F56_02205) 466049..466630 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
I8F56_RS02210 (I8F56_02210) 466995..467570 + 576 WP_002331662.1 SOS response-associated peptidase family protein -
I8F56_RS02215 (I8F56_02215) 467775..468113 - 339 WP_002286804.1 hypothetical protein -
I8F56_RS02220 (I8F56_02220) 468401..468631 + 231 WP_002323011.1 hypothetical protein Antitoxin
I8F56_RS02225 (I8F56_02225) 468631..468972 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I8F56_RS02230 (I8F56_02230) 469822..470007 + 186 WP_002304207.1 hypothetical protein -
I8F56_RS02235 (I8F56_02235) 470512..470706 + 195 WP_002295146.1 hypothetical protein -
I8F56_RS02240 (I8F56_02240) 470897..471146 + 250 Protein_441 transposase -
I8F56_RS02245 (I8F56_02245) 471390..472220 - 831 WP_002286789.1 manganese catalase family protein -
I8F56_RS02250 (I8F56_02250) 472428..473762 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T183331 WP_002302307.1 NZ_CP065767:468631-468972 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T183331 NZ_CP086901:c2251135-2250872 [Pseudosulfitobacter pseudonitzschiae]
ATGAAGTTCGAGTATGACCCTGACAAGAGTGCCGGGAACAAGGCCAAGCACGGCATCGACTTCGATGAAGCGCAAGCGCT
TTGGGAAGACCCGTGGCTGTTGGAGGCTCCCGCTCGAACAGATGACGAGCCGCGCTTTTTTGCAATAGGCATGATCAAGG
AACGGCATTGGTCAGCAGTTTGCACCTATCGCGGTGATATTATTCGGATCATCTCGGTGCGCCGCGCCAGAAAACAGGAG
ATTGAGCACTATGAAAGCACGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT183331 WP_002323011.1 NZ_CP065767:468401-468631 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT183331 NZ_CP086901:c2250885-2250670 [Pseudosulfitobacter pseudonitzschiae]
ATGAAAGCACGTGAGTTCGATCAAAAATTTGACACAGGTGAGAACGTCAGCGATCTTGTGGACTGGTCCAAAGCTAGGCG
GACGAATACCCAGACGAAGCGAGTCAATGTGGATTTTCCGTCGTGGGTCGTTTCTGGTTTGGATCAGAAGGCAAAGCAGC
TCGGCGTTACTCGTCAGTCGCTCATCAAACTCTGGATCGCCGAGCGGCTCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References