Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 34741..35348 Replicon plasmid p18-276_2
Accession NZ_CP065756
Organism Enterococcus faecium strain 18-276

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag I8F55_RS13920 Protein ID WP_002321032.1
Coordinates 34998..35348 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag I8F55_RS13915 Protein ID WP_002287514.1
Coordinates 34741..35004 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8F55_RS13880 (I8F55_13885) 30052..30312 + 261 WP_002296240.1 hypothetical protein -
I8F55_RS13885 (I8F55_13890) 30757..30963 + 207 WP_002325537.1 hypothetical protein -
I8F55_RS13890 (I8F55_13895) 30984..31214 + 231 WP_010726736.1 hypothetical protein -
I8F55_RS13895 (I8F55_13900) 31230..31667 + 438 WP_002332738.1 hypothetical protein -
I8F55_RS13900 (I8F55_13905) 31660..32367 + 708 WP_002332739.1 hypothetical protein -
I8F55_RS13905 (I8F55_13910) 32747..32926 + 180 WP_002305761.1 hypothetical protein -
I8F55_RS13910 (I8F55_13915) 33260..34447 - 1188 WP_002296840.1 IS256-like element IS16 family transposase -
I8F55_RS13915 (I8F55_13920) 34741..35004 + 264 WP_002287514.1 hypothetical protein Antitoxin
I8F55_RS13920 (I8F55_13925) 34998..35348 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I8F55_RS13925 (I8F55_13930) 35372..35986 - 615 WP_002318230.1 recombinase family protein -
I8F55_RS13930 (I8F55_13935) 36300..36722 + 423 WP_002288787.1 hypothetical protein -
I8F55_RS13935 (I8F55_13940) 36732..36935 + 204 WP_002299573.1 hypothetical protein -
I8F55_RS13940 (I8F55_13945) 37146..37730 + 585 WP_002299575.1 recombinase family protein -
I8F55_RS13945 (I8F55_13950) 38101..39426 + 1326 WP_071245491.1 Y-family DNA polymerase -
I8F55_RS13950 (I8F55_13955) 39419..39769 + 351 WP_112019300.1 hypothetical protein -
I8F55_RS13955 (I8F55_13960) 39766..39945 + 180 WP_071244496.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / VanHAX - 1..56729 56729
- flank IS/Tn - - 33260..34447 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T183329 WP_002321032.1 NZ_CP065756:34998-35348 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T183329 NZ_CP086900:c225765-225439 [Pseudosulfitobacter pseudonitzschiae]
GTGAGGCGTGGTGAGCTGGTGACGGTCGCCCTTCAGGGCGACCACGGCAAGCCGCGTCCCGCGCTTGTGATCCAATCCGA
CCGGTTTCCGGACACCGCCACCACGACCGTCCTTCTGGTTACATCCACCTTGGTGGATGCGCCGTTGATTCGGCTGACGG
TCGAACCCTCTGCGGAGAACGGGTTGCGTATGCGATCACAGATCATGATCGACAAAGCCGTGACAGTTCGAACGGACAAG
CTGGGGAAAGCTTTCGGTCAGCTGGACGATACCGCCATGATCGAGGTGAACCGGGCACTAGCACTGTTTTTAGGAGTGGC
GGGGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT183329 WP_002287514.1 NZ_CP065756:34741-35004 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT183329 NZ_CP086900:c225980-225762 [Pseudosulfitobacter pseudonitzschiae]
ATGCCAACCTCTGTTTCTCACCGTGTCCAGAAGCGTCGCGATGCCTTGCGTGCCGCTGGTTTGCGCCCCGTCCAGATTTG
GGTGCCGGATACCCGCCGCCCCGGTTTCGAGGCTGAGTGCCGCCGCCAGGCTGCAATTGTAGCCAAGGCCGATCGCGCAG
ATGATACTCTGAACGATTTCCTGGATGCCTCCCTGGTCGATGTGGATGACTGGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References