183328

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 479763..480334 Replicon chromosome
Accession NZ_CP065754
Organism Enterococcus faecium strain 18-276

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag I8F55_RS02285 Protein ID WP_002286801.1
Coordinates 479993..480334 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag I8F55_RS02280 Protein ID WP_002323011.1
Coordinates 479763..479993 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8F55_RS02250 (I8F55_02250) 474896..476227 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
I8F55_RS02255 (I8F55_02255) 476249..476875 + 627 WP_002286816.1 cysteine hydrolase -
I8F55_RS02260 (I8F55_02260) 477058..477639 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
I8F55_RS14400 478371..478933 + 563 Protein_450 SOS response-associated peptidase -
I8F55_RS02275 (I8F55_02275) 479138..479476 - 339 WP_002286804.1 hypothetical protein -
I8F55_RS02280 (I8F55_02280) 479763..479993 + 231 WP_002323011.1 hypothetical protein Antitoxin
I8F55_RS02285 (I8F55_02285) 479993..480334 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I8F55_RS02290 (I8F55_02290) 481184..481369 + 186 WP_002304207.1 hypothetical protein -
I8F55_RS02295 (I8F55_02295) 481874..482068 + 195 WP_002297028.1 hypothetical protein -
I8F55_RS02300 (I8F55_02300) 482259..482508 + 250 Protein_456 transposase -
I8F55_RS02305 (I8F55_02305) 482752..483582 - 831 WP_002286789.1 manganese catalase family protein -
I8F55_RS02310 (I8F55_02310) 483790..485124 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T183328 WP_002286801.1 NZ_CP065754:479993-480334 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T183328 NZ_CP086900:120568-120990 [Pseudosulfitobacter pseudonitzschiae]
ATGATCATCCTCGATACGAATGTCATCTGCGAGCTGTTGCGCCCGACCCCGGAGCCCAGGATCGAGCAATGGCTGTCCGC
TCAAGATGGTCTAAACGTCTACCTGACCTCGATATCGGAGGCGGAGCTGCGCTATGGCCTCGCGAACATGGGGAACGGCA
AGCGCCGCGCTGCGCTGATTGACGCCGTAGACCGCATCCTGCGCGAGGACCTCGCCGGGCGCATTCTGCCCTTCGACAGC
GACGCCGCGCAGGCATACGCCTCTATCGCCGCCACTCGTCGCGACGCCGGGCGACCGATTGCCCAGGCCGATTGCCAGAT
CGCATCCATTGCCCACGCCTGTGGCGCCACCGTCGCCACACGCAATACGCCCGACTTCGAGGGTTGCGAGATTGACCTGA
TCAACCCCTGGACAACCGAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT183328 WP_002323011.1 NZ_CP065754:479763-479993 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT183328 NZ_CP086900:120316-120564 [Pseudosulfitobacter pseudonitzschiae]
ATGGCAAGCATTACGATCCGGAACCTTGACGATGACGTGAAGCGGCGCCTTCGCATCCGAGCGGCCGAGCATGGCCGTTC
GATGGAAGAGGAGGCGCGAGAGATCCTGCGCCAAGTCGTTGGCGACGCGAAGCCCAACCATGATCTTGCCGCGGCGATCC
GTGCGCGGGTTGGACCCTTGGGTGGCGTTGAACTTGACCTTCCGCAGCGTGAAGCCATGCATGAGCCGCCCACATTCGAC
CGAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References