Detailed information of TA system TA183326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 512983..513554
• Replicon: chromosome
• Accession: NZ_CP065752
• Organism: Enterococcus faecium strain 18-465

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I8F49_RS02510
• Protein ID: WP_002328559.1
• Coordinates: 513213..513554 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: I8F49_RS02505
• Protein ID: WP_002323011.1
• Coordinates: 512983..513213 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8F49_RS02480 (I8F49_02470)	508446..509774	+	1329	WP_002327663.1	FAD-containing oxidoreductase	-
I8F49_RS02485 (I8F49_02475)	509796..510422	+	627	WP_002286816.1	cysteine hydrolase	-
I8F49_RS02490 (I8F49_02480)	510605..511186	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
I8F49_RS02495 (I8F49_02485)	511573..512148	+	576	WP_002327664.1	SOS response-associated peptidase family protein	-
I8F49_RS02500 (I8F49_02490)	512349..512687	-	339	WP_002327665.1	hypothetical protein	-
I8F49_RS02505 (I8F49_02495)	512983..513213	+	231	WP_002323011.1	hypothetical protein	Antitoxin
I8F49_RS02510 (I8F49_02500)	513213..513554	+	342	WP_002328559.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I8F49_RS02515 (I8F49_02505)	514373..515535	+	1163	WP_143351449.1	IS3 family transposase	-
I8F49_RS02520 (I8F49_02510)	515666..516850	+	1185	Protein_504	SpaA isopeptide-forming pilin-related protein	-
I8F49_RS02525 (I8F49_02515)	516850..518163	+	1314	WP_002328556.1	pilin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13278.70 Da
• Isoelectric Point: 10.1461

>T183326 WP_002328559.1 NZ_CP065752:513213-513554 [Enterococcus faecium]
VSGERIYIPKKGDIVWIYFDPSLGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T183326 NZ_CP086896:92775-93041 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT183326 WP_002323011.1 NZ_CP065752:512983-513213 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT183326 NZ_CP086896:92527-92778 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9