Detailed information of TA system TA183320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 355673..356307
• Replicon: chromosome
• Accession: NZ_CP065750
• Organism: Pseudomonas stutzeri strain FDAARGOS_875

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6G32_RS01515
• Protein ID: WP_003291451.1
• Coordinates: 355673..356077 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L1LS63
• Locus tag: I6G32_RS01520
• Protein ID: WP_003285520.1
• Coordinates: 356077..356307 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G32_RS01490	350842..351933	-	1092	WP_157782031.1	inovirus-type Gp2 protein	-
I6G32_RS01495	352154..353653	-	1500	WP_003291448.1	DUF3987 domain-containing protein	-
I6G32_RS01500	353706..353909	-	204	WP_003291449.1	AlpA family phage regulatory protein	-
I6G32_RS01505	354229..354561	+	333	WP_036992178.1	hypothetical protein	-
I6G32_RS01510	354621..355589	+	969	WP_003291450.1	DUF932 domain-containing protein	-
I6G32_RS01515	355673..356077	-	405	WP_003291451.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I6G32_RS01520	356077..356307	-	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6G32_RS01525	356464..357468	+	1005	WP_003291452.1	YqaJ viral recombinase family protein	-
I6G32_RS01530	357560..358510	+	951	WP_013982991.1	hypothetical protein	-
I6G32_RS01535	358482..358979	+	498	WP_013982992.1	DNA repair protein RadC	-
I6G32_RS01540	358989..359156	+	168	WP_003291475.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14968.21 Da
• Isoelectric Point: 7.2774

>T183320 WP_003291451.1 NZ_CP065750:c356077-355673 [Pseudomonas stutzeri]
MLKYMLDTNMCIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVSA

Nucleotide

• Length: 405 bp

>T183320 NZ_CP086889:c80281-79859 [Pseudosulfitobacter pseudonitzschiae]
ATGATCATCCTCGATACGAATGTCATCTGCGAGCTGTTGCGCCCGACCCCGGAGCCCAGGATCGAGCAATGGCTGTCCGC
TCAAGATGGTCTAAACGTCTACCTGACCTCGATATCGGAGGCGGAGCTGCGCTATGGCCTCGCGAACATGGGGAACGGCA
AGCGCCGCGCTGCGCTGATTGACGCCGTAGACCGCATCCTGCGCGAGGACCTCGCCGGGCGCATTCTGCCCTTCGACAGC
GACGCCGCGCAGGCATACGCCTCTATCGCCGCCACTCGTCGCGACGCCGGGCGACCGATTGCCCAGGCCGATTGCCAGAT
CGCATCCATTGCCCACGCCTGTGGCGCCACCGTCGCCACACGCAATACGCCCGACTTCGAGGGTTGCGAGATTGACCTGA
TCAACCCCTGGACAACCGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8432.41 Da
• Isoelectric Point: 4.2683

>AT183320 WP_003285520.1 NZ_CP065750:c356307-356077 [Pseudomonas stutzeri]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT183320 NZ_CP086889:c80533-80285 [Pseudosulfitobacter pseudonitzschiae]
ATGGCAAGCATTACGATCCGGAACCTTGACGATGACGTGAAGCGGCGCCTTCGCATCCGAGCGGCCGAGCATGGCCGTTC
GATGGAAGAGGAGGCGCGAGAGATCCTGCGCCAAGTCGTTGGCGACGCGAAGCCCAACCATGATCTTGCCGCGGCGATCC
GTGCGCGGGTTGGACCCTTGGGTGGCGTTGAACTTGACCTTCCGCAGCGTGAAGCCATGCATGAGCCGCCCACATTCGAC
CGAGCCTAA

Structures

Antitoxin

• AlphaFold DB: L1LS63