Detailed information of TA system TA183315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 3456616..3457162
• Replicon: chromosome
• Accession: NZ_CP065748
• Organism: Delftia lacustris strain FDAARGOS_890

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: I6G47_RS16135
• Protein ID: WP_133073840.1
• Coordinates: 3456616..3456882 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6G47_RS16140
• Protein ID: WP_133073839.1
• Coordinates: 3456869..3457162 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G47_RS16110	3451992..3452366	+	375	WP_133073843.1	phage tail assembly protein	-
I6G47_RS16115	3452375..3452500	+	126	WP_096915334.1	GpE family phage tail protein	-
I6G47_RS16120	3452493..3455000	+	2508	WP_133073842.1	phage tail tape measure protein	-
I6G47_RS16125	3455013..3455477	+	465	WP_096915332.1	phage tail protein	-
I6G47_RS16130	3455474..3456562	+	1089	WP_133073841.1	phage late control D family protein	-
I6G47_RS16135	3456616..3456882	+	267	WP_133073840.1	BrnT family toxin	Toxin
I6G47_RS16140	3456869..3457162	+	294	WP_133073839.1	BrnA antitoxin family protein	Antitoxin
I6G47_RS16145	3457226..3457528	+	303	WP_133073838.1	hypothetical protein	-
I6G47_RS16150	3457525..3457767	+	243	WP_133073837.1	hypothetical protein	-
I6G47_RS16155	3457764..3458171	+	408	WP_133073836.1	hypothetical protein	-
I6G47_RS16160	3458220..3458744	-	525	WP_133073835.1	hypothetical protein	-
I6G47_RS16165	3458777..3459349	-	573	WP_133073834.1	helix-turn-helix domain-containing protein	-
I6G47_RS16170	3459430..3459678	+	249	WP_133073833.1	hypothetical protein	-
I6G47_RS16175	3459875..3460231	+	357	WP_133073832.1	ogr/Delta-like zinc finger family protein	-
I6G47_RS16180	3460346..3460756	+	411	WP_133073831.1	hypothetical protein	-
I6G47_RS16185	3460753..3460980	+	228	WP_133073830.1	hypothetical protein	-
I6G47_RS16190	3460977..3461135	+	159	WP_189354251.1	hypothetical protein	-
I6G47_RS16195	3461132..3461545	+	414	WP_133073829.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3432876..3509351	76475

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (30-90)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10140.63 Da
• Isoelectric Point: 6.4616

>T183315 WP_133073840.1 NZ_CP065748:3456616-3456882 [Delftia lacustris]
MDIEFDPAKNQANIDKHGVSLEMAGHIEWSNVLCMPDARRDYGEVREIGFTVIGQRLYVVVFVQREQTMRIVSLRKANSR
EVKAYEAI

Nucleotide

• Length: 267 bp

>T183315 NZ_CP086886:1830118-1830372 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10556.32 Da
• Isoelectric Point: 9.9469

>AT183315 WP_133073839.1 NZ_CP065748:3456869-3457162 [Delftia lacustris]
MKQSKKVGLIQPTAAEDAAIARGIEQDPDTMEITGDMLADMQPLVRRGRPPLEQPKMPMTMRVDADVLEAIKATGTGWQS
RVNSVLREAVKKGKLAA

Nucleotide

• Length: 294 bp

>AT183315 NZ_CP086886:1830369-1830698 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA