Detailed information of TA system TA183314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2396740..2397286
• Replicon: chromosome
• Accession: NZ_CP065748
• Organism: Delftia lacustris strain FDAARGOS_890

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: I6G47_RS11200
• Protein ID: WP_198129381.1
• Coordinates: 2396740..2397006 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6G47_RS11205
• Protein ID: WP_198129382.1
• Coordinates: 2396993..2397286 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G47_RS11175	2392070..2392501	+	432	WP_198129376.1	phage tail assembly protein	-
I6G47_RS11180	2392510..2392635	+	126	WP_198129377.1	GpE family phage tail protein	-
I6G47_RS11185	2392628..2395135	+	2508	WP_198129378.1	phage tail tape measure protein	-
I6G47_RS11190	2395146..2395607	+	462	WP_198129379.1	phage tail protein	-
I6G47_RS11195	2395607..2396686	+	1080	WP_198129380.1	phage late control D family protein	-
I6G47_RS11200	2396740..2397006	+	267	WP_198129381.1	BrnT family toxin	Toxin
I6G47_RS11205	2396993..2397286	+	294	WP_198129382.1	BrnA antitoxin family protein	Antitoxin
I6G47_RS11210	2397350..2397652	+	303	WP_133073838.1	hypothetical protein	-
I6G47_RS11215	2397649..2397885	+	237	WP_198129383.1	hypothetical protein	-
I6G47_RS11220	2397923..2398144	-	222	WP_198129384.1	hypothetical protein	-
I6G47_RS11225	2398131..2398493	-	363	WP_198129385.1	hypothetical protein	-
I6G47_RS11230	2398698..2399645	-	948	WP_198129386.1	WYL domain-containing protein	-
I6G47_RS11235	2399697..2400260	-	564	WP_198129387.1	transcriptional regulator	-
I6G47_RS11240	2400341..2400589	+	249	WP_198129388.1	hypothetical protein	-
I6G47_RS11245	2400786..2401142	+	357	WP_133073832.1	ogr/Delta-like zinc finger family protein	-
I6G47_RS11250	2401257..2401667	+	411	WP_133073831.1	hypothetical protein	-
I6G47_RS11255	2401664..2401891	+	228	WP_133073830.1	hypothetical protein	-
I6G47_RS11260	2401888..2402046	+	159	WP_189354251.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2370009..2409520	39511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (29-90)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10039.52 Da
• Isoelectric Point: 6.4605

>T183314 WP_198129381.1 NZ_CP065748:2396740-2397006 [Delftia lacustris]
MDIEFDPAKNQANIDKHGVSLEMAGHIEWSDVLCMPDARRDYGEVREIGFAVIGQRLYVVVFVQRGQTMRIVSLRKANSR
EVKAYEAI

Nucleotide

• Length: 267 bp

>T183314 NZ_CP086885:11630-11905 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10486.22 Da
• Isoelectric Point: 9.9469

>AT183314 WP_198129382.1 NZ_CP065748:2396993-2397286 [Delftia lacustris]
MKQSKKVGLIQPTAAEDAAIARGIEQDPDTMEITGDMLAGMQTLARRGRPPLEQPKMPMTMRVDADVLEAIKATGAGWQS
RVNEVLREAVKKGKLAA

Nucleotide

• Length: 294 bp

>AT183314 NZ_CP086885:11344-11637 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA