Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1136912..1137554 Replicon chromosome
Accession NZ_CP065743
Organism Bacillus tropicus strain FDAARGOS_897

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag I6G54_RS07250 Protein ID WP_000635963.1
Coordinates 1137204..1137554 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag I6G54_RS07245 Protein ID WP_000004570.1
Coordinates 1136912..1137199 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G54_RS07220 1132229..1133191 + 963 WP_000961159.1 UV DNA damage repair endonuclease UvsE -
I6G54_RS07225 1133184..1133756 - 573 WP_001975497.1 rhomboid family intramembrane serine protease -
I6G54_RS07230 1133849..1134208 + 360 WP_000635047.1 holo-ACP synthase -
I6G54_RS07235 1134365..1135315 + 951 WP_033691678.1 outer membrane lipoprotein carrier protein LolA -
I6G54_RS07240 1135433..1136602 + 1170 WP_000390607.1 alanine racemase -
I6G54_RS07245 1136912..1137199 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
I6G54_RS07250 1137204..1137554 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I6G54_RS07255 1137622..1139790 + 2169 WP_001975517.1 RNA-binding transcriptional accessory protein -
I6G54_RS07260 1139848..1139964 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
I6G54_RS07265 1140160..1140618 + 459 WP_001975527.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T183293 WP_000635963.1 NZ_CP065743:1137204-1137554 [Bacillus tropicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T183293 NZ_CP086863:98070-98492 [Pseudosulfitobacter pseudonitzschiae]
ATGATCATCCTCGATACGAATGTCATCTGCGAGCTGTTGCGCCCGACCCCGGAGCCCAGGATCGAGCAATGGCTGTCCGC
TCAAGATGGTCTAAACGTCTACCTGACCTCGATATCGGAGGCGGAGCTGCGCTATGGCCTCGCGAACATGGGGAACGGCA
AGCGCCGCGCTGCGCTGATTGACGCCGTAGACCGCATCCTGCGCGAGGACCTCGCCGGGCGCATTCTGCCCTTCGACAGC
GACGCCGCGCAGGCATACGCCTCTATCGCCGCCACTCGTCGCGACGCCGGGCGACCGATTGCCCAGGCCGATTGCCAGAT
CGCATCCATTGCCCACGCCTGTGGCGCCACCGTCGCCACACGCAATACGCCCGACTTCGAGGGTTGCGAGATTGACCTGA
TCAACCCCTGGACAACCGAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT183293 WP_000004570.1 NZ_CP065743:1136912-1137199 [Bacillus tropicus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT183293 NZ_CP086863:97818-98066 [Pseudosulfitobacter pseudonitzschiae]
ATGGCAAGCATTACGATCCGGAACCTTGACGATGACGTGAAGCGGCGCCTTCGCATCCGAGCGGCCGAGCATGGCCGTTC
GATGGAAGAGGAGGCGCGAGAGATCCTGCGCCAAGTCGTTGGCGACGCGAAGCCCAACCATGATCTTGCCGCGGCGATCC
GTGCGCGGGTTGGACCCTTGGGTGGCGTTGAACTTGACCTTCCGCAGCGTGAAGCCATGCATGAGCCGCCCACATTCGAC
CGAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References