Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4090280..4090922 Replicon chromosome
Accession NZ_CP065739
Organism Bacillus tropicus strain FDAARGOS_920

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag I6G77_RS21210 Protein ID WP_000635963.1
Coordinates 4090572..4090922 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag I6G77_RS21205 Protein ID WP_000004570.1
Coordinates 4090280..4090567 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G77_RS21180 4085597..4086559 + 963 WP_042515799.1 UV DNA damage repair endonuclease UvsE -
I6G77_RS21185 4086552..4087124 - 573 WP_001975497.1 rhomboid family intramembrane serine protease -
I6G77_RS21190 4087217..4087576 + 360 WP_000635047.1 holo-ACP synthase -
I6G77_RS21195 4087733..4088683 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
I6G77_RS21200 4088801..4089970 + 1170 WP_000390607.1 alanine racemase -
I6G77_RS21205 4090280..4090567 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
I6G77_RS21210 4090572..4090922 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I6G77_RS21215 4090990..4093158 + 2169 WP_001975517.1 RNA-binding transcriptional accessory protein -
I6G77_RS21220 4093216..4093332 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
I6G77_RS21225 4093528..4093986 + 459 WP_001975527.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T183292 WP_000635963.1 NZ_CP065739:4090572-4090922 [Bacillus tropicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T183292 NZ_CP086862:3423163-3423417 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT183292 WP_000004570.1 NZ_CP065739:4090280-4090567 [Bacillus tropicus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT183292 NZ_CP086862:3423414-3423743 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References