Detailed information of TA system TA183283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/HTH(antitoxin)
• Location: 1275109..1275514
• Replicon: chromosome
• Accession: NZ_CP065725
• Organism: Oligella ureolytica strain FDAARGOS_872

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: I6G29_RS06390
• Protein ID: WP_018575637.1
• Coordinates: 1275109..1275306 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A378XGM7
• Locus tag: I6G29_RS06395
• Protein ID: WP_018575636.1
• Coordinates: 1275296..1275514 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G29_RS06360	1270240..1270428	+	189	WP_018575640.1	DUF4926 domain-containing protein	-
I6G29_RS06365	1270523..1271776	+	1254	WP_018575721.1	IS256 family transposase	-
I6G29_RS06370	1271833..1272189	+	357	WP_115148548.1	ATP-binding protein	-
I6G29_RS06375	1272446..1273456	-	1011	WP_115061735.1	IS5 family transposase	-
I6G29_RS06380	1273681..1274691	-	1011	WP_115061735.1	IS5 family transposase	-
I6G29_RS06385	1274880..1275086	+	207	WP_197933430.1	hypothetical protein	-
I6G29_RS06390	1275109..1275306	-	198	WP_018575637.1	hypothetical protein	Toxin
I6G29_RS06395	1275296..1275514	-	219	WP_018575636.1	TraY domain-containing protein	Antitoxin
I6G29_RS06400	1275617..1275895	-	279	WP_018575635.1	HigA family addiction module antidote protein	-
I6G29_RS06405	1275906..1276184	-	279	WP_018027459.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6G29_RS06410	1276370..1277514	+	1145	WP_115062989.1	IS3 family transposase	-
I6G29_RS06415	1277790..1279009	+	1220	WP_115061740.1	IS3 family transposase	-
I6G29_RS06420	1279368..1279805	-	438	WP_018575206.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	blaCARB-5	-	1263226..1279009	15783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Relaxosome protein TraY (4-48)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7773.17 Da
• Isoelectric Point: 10.9229

>T183283 WP_018575637.1 NZ_CP065725:c1275306-1275109 [Oligella ureolytica]
MTYKILYSDKAKKALLKLDKPVAKRIHRFLTERVSQDPKNQGGFLQGNFWRREFIKEFNLSVFII

Nucleotide

• Length: 198 bp

>T183283 NZ_CP086854:1683159-1683413 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8235.44 Da
• Isoelectric Point: 4.8839

>AT183283 WP_018575636.1 NZ_CP065725:c1275514-1275296 [Oligella ureolytica]
MLAIRLPDEIDNRLKDLAEKTGRTKSFYAREAILKHLDDLEGIYLAERELELIKAGKSKTYSAEEVGKELDL

Nucleotide

• Length: 219 bp

>AT183283 NZ_CP086854:1683410-1683739 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA

Structures

Antitoxin

• AlphaFold DB: A0A378XGM7