Detailed information of TA system TA183267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1296194..1296825
• Replicon: chromosome
• Accession: NZ_CP065717
• Organism: Serratia rubidaea strain FDAARGOS_880

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: L0MF14
• Locus tag: I6G37_RS06175
• Protein ID: WP_015671220.1
• Coordinates: 1296194..1296397 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: I6G37_RS06180
• Protein ID: WP_105230044.1
• Coordinates: 1296457..1296825 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G37_RS06155	1291390..1291812	+	423	WP_187043541.1	hypothetical protein	-
I6G37_RS06160	1291847..1293190	-	1344	WP_111737623.1	NCS2 family permease	-
I6G37_RS06165	1293260..1295047	-	1788	WP_197951397.1	amidohydrolase family protein	-
I6G37_RS06170	1295186..1296118	+	933	WP_187043539.1	LysR family transcriptional regulator	-
I6G37_RS06175	1296194..1296397	-	204	WP_015671220.1	hemolysin expression modulator Hha	Toxin
I6G37_RS06180	1296457..1296825	-	369	WP_105230044.1	Hha toxicity modulator TomB	Antitoxin
I6G37_RS06185	1297201..1297554	-	354	WP_197951399.1	hypothetical protein	-
I6G37_RS06190	1297812..1297958	+	147	WP_158684784.1	hypothetical protein	-
I6G37_RS06195	1297955..1298659	+	705	WP_197951401.1	ABC transporter ATP-binding protein	-
I6G37_RS06200	1298656..1299522	+	867	WP_105230047.1	metal ABC transporter permease	-
I6G37_RS06205	1299538..1300413	+	876	WP_105230048.1	metal ABC transporter substrate-binding protein	-
I6G37_RS06210	1300512..1300652	-	141	WP_004940304.1	type B 50S ribosomal protein L36	-
I6G37_RS06215	1300662..1300916	-	255	WP_015671227.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8049.33 Da
• Isoelectric Point: 7.9816

>T183267 WP_015671220.1 NZ_CP065717:c1296397-1296194 [Serratia rubidaea]
MTKTDYLMRLRKCTTIDTLERVIEKNKYALSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Nucleotide

• Length: 204 bp

>T183267 NZ_CP086839:c378947-378693 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14350.17 Da
• Isoelectric Point: 4.6346

>AT183267 WP_105230044.1 NZ_CP065717:c1296825-1296457 [Serratia rubidaea]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSYKIKYMDESDFSELVEEYL
DDTYTLFSNYGINDSDLRRWQKTKLRLFRMFSGEVCCTKMKT

Nucleotide

• Length: 369 bp

>AT183267 NZ_CP086839:c378696-378367 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA

Structures

Toxin

• AlphaFold DB: A0A857EAE6