Detailed information of TA system TA183265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 264685..265358
• Replicon: plasmid unnamed1
• Accession: NZ_CP065714
• Organism: Sphingomonas paucimobilis strain FDAARGOS_881

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6G38_RS20465
• Protein ID: WP_042469253.1
• Coordinates: 264945..265358 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0C9NIS3
• Locus tag: I6G38_RS20460
• Protein ID: WP_042469255.1
• Coordinates: 264685..264948 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G38_RS20420	260401..260733	+	333	WP_042469261.1	DUF3768 domain-containing protein	-
I6G38_RS20425	260867..261061	+	195	WP_042469259.1	hypothetical protein	-
I6G38_RS20430	261058..261387	+	330	WP_042469258.1	hypothetical protein	-
I6G38_RS20435	261528..262025	+	498	WP_042469256.1	hypothetical protein	-
I6G38_RS20440	261991..262461	-	471	WP_156146122.1	hypothetical protein	-
I6G38_RS20445	262772..263089	+	318	WP_042469311.1	hypothetical protein	-
I6G38_RS20450	263266..263829	+	564	WP_042469310.1	MucR family transcriptional regulator	-
I6G38_RS20455	263993..264496	+	504	WP_030541254.1	DUF736 domain-containing protein	-
I6G38_RS20460	264685..264948	+	264	WP_042469255.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6G38_RS20465	264945..265358	+	414	WP_042469253.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6G38_RS20470	265455..266657	+	1203	WP_082052518.1	SEC-C domain-containing protein	-
I6G38_RS20475	266918..267238	+	321	WP_042469252.1	single-stranded DNA-binding protein	-
I6G38_RS20480	267288..268400	-	1113	WP_042469251.1	hypothetical protein	-
I6G38_RS20485	268882..269859	+	978	WP_030541261.1	DUF2493 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..426536	426536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (16-53)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14397.54 Da
• Isoelectric Point: 5.6141

>T183265 WP_042469253.1 NZ_CP065714:264945-265358 [Sphingomonas paucimobilis]
VTQLYMLDTNIVSELARNPHGAVATRIAQVGADAICVSIITAAELRYGCAKKGSPRLLAQIEAILGSVSVLALDVPADTE
YGGIRAELESAGKPIGPNDLFIAAHAYALGATLVTANIGEFSRVRALKVENWLSDVH

Nucleotide

• Length: 414 bp

>T183265 NZ_CP086836:c131785-131363 [Pseudosulfitobacter pseudonitzschiae]
ATGATCATCCTCGATACGAATGTCATCTGCGAGCTGTTGCGCCCGACCCCGGAGCCCAGGATCGAGCAATGGCTGTCCGC
TCAAGATGGTCTAAACGTCTACCTGACCTCGATATCGGAGGCGGAGCTGCGCTATGGCCTCGCGAACATGGGGAACGGCA
AGCGCCGCGCTGCGCTGATTGACGCCGTAGACCGCATCCTGCGCGAGGACCTCGCCGGGCGCATTCTGCCCTTCGACAGC
GACGCCGCGCAGGCATACGCCTCTATCGCCGCCACTCGTCGCGACGCCGGGCGACCGATTGCCCAGGCCGATTGCCAGAT
CGCATCCATTGCCCACGCCTGTGGCGCCACCGTCGCCACACGCAATACGCCCGACTTCGAGGGTTGCGAGATTGACCTGA
TCAACCCCTGGACAACCGAATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9794.17 Da
• Isoelectric Point: 4.7010

>AT183265 WP_042469255.1 NZ_CP065714:264685-264948 [Sphingomonas paucimobilis]
VTPDHEATVTREAKLFRNNKSQAVRIPADFELPGDRVMIHREGDRLVIEPIRRRNIVEVLASLEPLGPEDEFPDIDGTLL
PAKAVEL

Nucleotide

• Length: 264 bp

>AT183265 NZ_CP086836:c132037-131789 [Pseudosulfitobacter pseudonitzschiae]
ATGGCAAGCATTACGATCCGGAACCTTGACGATGACGTGAAGCGGCGCCTTCGCATCCGAGCGGCCGAGCATGGCCGTTC
GATGGAAGAGGAGGCGCGAGAGATCCTGCGCCAAGTCGTTGGCGACGCGAAGCCCAACCATGATCTTGCCGCGGCGATCC
GTGCGCGGGTTGGACCCTTGGGTGGCGTTGAACTTGACCTTCCGCAGCGTGAAGCCATGCATGAGCCGCCCACATTCGAC
CGAGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0C9NIS3