Detailed information of TA system TA183232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2181026..2181579
• Replicon: chromosome
• Accession: NZ_CP065699
• Organism: Serratia plymuthica strain FDAARGOS_889

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S4YH80
• Locus tag: I6G46_RS10245
• Protein ID: WP_020438747.1
• Coordinates: 2181026..2181340 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S4YG56
• Locus tag: I6G46_RS10250
• Protein ID: WP_020438746.1
• Coordinates: 2181343..2181579 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G46_RS10225	2176794..2177951	-	1158	WP_006324392.1	YbfB/YjiJ family MFS transporter	-
I6G46_RS10230	2178059..2178976	+	918	WP_004942342.1	LysR family transcriptional regulator	-
I6G46_RS10235	2179005..2180132	+	1128	WP_004942340.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
I6G46_RS10240	2180147..2180992	+	846	WP_006324390.1	S-formylglutathione hydrolase	-
I6G46_RS10245	2181026..2181340	-	315	WP_020438747.1	CcdB family protein	Toxin
I6G46_RS10250	2181343..2181579	-	237	WP_020438746.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
I6G46_RS10255	2181724..2182632	-	909	WP_004942335.1	glutathione ABC transporter permease GsiD	-
I6G46_RS10260	2182642..2183562	-	921	WP_006324386.1	glutathione ABC transporter permease GsiC	-
I6G46_RS10265	2183615..2185153	-	1539	WP_006324385.1	glutathione ABC transporter substrate-binding protein GsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11685.58 Da
• Isoelectric Point: 5.8036

>T183232 WP_020438747.1 NZ_CP065699:c2181340-2181026 [Serratia plymuthica]
MQFTVYANTGKSAVYPLLLDVTSDIIGQLNRRVVIPLLPIEKYPASTRPERLNPLVRLVDDNEYAVMTHEMASIPVRILG
AEFCDASQYRTKVKAAIDFLFDGI

Nucleotide

• Length: 315 bp

>T183232 NZ_CP086802:143014-143436 [Pseudosulfitobacter pseudonitzschiae]
ATGATCATCCTCGATACGAATGTCATCTGCGAGCTGTTGCGCCCGACCCCGGAGCCCAGGATCGAGCAATGGCTGTCCGC
TCAAGATGGTCTAAACGTCTACCTGACCTCGATATCGGAGGCGGAGCTGCGCTATGGCCTCGCGAACATGGGGAACGGCA
AGCGCCGCGCTGCGCTGATTGACGCCGTAGACCGCATCCTGCGCGAGGACCTCGCCGGGCGCATTCTGCCCTTCGACAGC
GACGCCGCGCAGGCATACGCCTCTATCGCCGCCACTCGTCGCGACGCCGGGCGACCGATTGCCCAGGCCGATTGCCAGAT
CGCATCCATTGCCCACGCCTGTGGCGCCACCGTCGCCACACGCAATACGCCCGACTTCGAGGGTTGCGAGATTGACCTGA
TCAACCCCTGGACAACCGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9078.16 Da
• Isoelectric Point: 6.7184

>AT183232 WP_020438746.1 NZ_CP065699:c2181579-2181343 [Serratia plymuthica]
MPVKQRTTQSVTMAVERSLLSRAREAGINLSATLNAALDAELRQHEMRKWKEENREAIDALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT183232 NZ_CP086802:142762-143010 [Pseudosulfitobacter pseudonitzschiae]
ATGGCAAGCATTACGATCCGGAACCTTGACGATGACGTGAAGCGGCGCCTTCGCATCCGAGCGGCCGAGCATGGCCGTTC
GATGGAAGAGGAGGCGCGAGAGATCCTGCGCCAAGTCGTTGGCGACGCGAAGCCCAACCATGATCTTGCCGCGGCGATCC
GTGCGCGGGTTGGACCCTTGGGTGGCGTTGAACTTGACCTTCCGCAGCGTGAAGCCATGCATGAGCCGCCCACATTCGAC
CGAGCCTAA

Structures

Toxin

• AlphaFold DB: S4YH80

Antitoxin

• AlphaFold DB: S4YG56