Detailed information of TA system TA183203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 945946..946562
• Replicon: chromosome
• Accession: NZ_CP065693
• Organism: Enterobacter asburiae strain FDAARGOS_892

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6G49_RS04795
• Protein ID: WP_081051446.1
• Coordinates: 946191..946562 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A376FEL2
• Locus tag: I6G49_RS04790
• Protein ID: WP_029741590.1
• Coordinates: 945946..946188 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G49_RS04760	941364..941834	+	471	WP_054829776.1	hypothetical protein	-
I6G49_RS04765	941923..942522	+	600	WP_029740331.1	glucose-1-phosphatase	-
I6G49_RS04770	942516..943397	+	882	WP_047646947.1	virulence factor BrkB family protein	-
I6G49_RS04775	943394..943831	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6G49_RS04780	943876..944817	+	942	WP_059347397.1	fatty acid biosynthesis protein FabY	-
I6G49_RS04785	944826..945746	-	921	WP_054829775.1	alpha/beta hydrolase	-
I6G49_RS04790	945946..946188	+	243	WP_029741590.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6G49_RS04795	946191..946562	+	372	WP_081051446.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6G49_RS04800	946602..947531	-	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
I6G49_RS04805	947528..948163	-	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
I6G49_RS04810	948160..949062	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13589.71 Da
• Isoelectric Point: 6.9789

>T183203 WP_081051446.1 NZ_CP065693:946191-946562 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTAAVMGAFEIIDISRDIAERSVLLR
EKHGMKLPDAIILATAQSRSCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T183203 NZ_CP086775:c1333095-1332841 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9401.51 Da
• Isoelectric Point: 4.1735

>AT183203 WP_029741590.1 NZ_CP065693:945946-946188 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT183203 NZ_CP086775:c1332844-1332515 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA

Structures

Antitoxin

• AlphaFold DB: A0A376FEL2