Detailed information of TA system TA183175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 54558..55207
• Replicon: plasmid unnamed
• Accession: NZ_CP065685
• Organism: Burkholderia humptydooensis strain FDAARGOS_899

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6G56_RS00245
• Protein ID: WP_006029599.1
• Coordinates: 54558..54968 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7U4P815
• Locus tag: I6G56_RS00250
• Protein ID: WP_006029598.1
• Coordinates: 54965..55207 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G56_RS00225	51658..52386	-	729	WP_009916772.1	hypothetical protein	-
I6G56_RS00230	52425..53192	-	768	WP_006029601.1	hypothetical protein	-
I6G56_RS00235	53490..53948	+	459	WP_006029600.1	hypothetical protein	-
I6G56_RS00240	54316..54480	-	165	WP_156436729.1	hypothetical protein	-
I6G56_RS00245	54558..54968	-	411	WP_006029599.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6G56_RS00250	54965..55207	-	243	WP_006029598.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6G56_RS00255	55715..56476	+	762	WP_144411849.1	hypothetical protein	-
I6G56_RS00260	56675..57643	-	969	WP_043283354.1	ParB/RepB/Spo0J family partition protein	-
I6G56_RS00265	57640..58848	-	1209	WP_009916766.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	bspR2 / bspI2	1..305114	305114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14767.04 Da
• Isoelectric Point: 5.1526

>T183175 WP_006029599.1 NZ_CP065685:c54968-54558 [Burkholderia humptydooensis]
MTLYLLDTNILSNVIRDPRGACTARIGETPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFARVAGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T183175 NZ_CP086759:c728564-728382 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8499

>AT183175 WP_006029598.1 NZ_CP065685:c55207-54965 [Burkholderia humptydooensis]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDETFPDIDNLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT183175 NZ_CP086759:c728289-727885 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4P815