Detailed information of TA system TA183158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1456099..1456721
• Replicon: chromosome
• Accession: NZ_CP065673
• Organism: Serratia plymuthica strain FDAARGOS_907

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: S4YCZ4
• Locus tag: I6G64_RS06970
• Protein ID: WP_006322256.1
• Coordinates: 1456518..1456721 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S4YKH5
• Locus tag: I6G64_RS06965
• Protein ID: WP_004949348.1
• Coordinates: 1456099..1456467 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G64_RS06935	1452140..1452394	+	255	WP_004949355.1	type B 50S ribosomal protein L31	-
I6G64_RS06940	1452407..1452547	+	141	WP_004949353.1	type B 50S ribosomal protein L36	-
I6G64_RS06945	1452663..1453541	-	879	WP_063198026.1	metal ABC transporter substrate-binding protein	-
I6G64_RS06950	1453567..1454424	-	858	WP_063198028.1	metal ABC transporter permease	-
I6G64_RS06955	1454421..1455134	-	714	WP_062869870.1	ABC transporter ATP-binding protein	-
I6G64_RS06960	1455586..1455939	+	354	WP_063198033.1	hypothetical protein	-
I6G64_RS06965	1456099..1456467	+	369	WP_004949348.1	Hha toxicity modulator TomB	Antitoxin
I6G64_RS06970	1456518..1456721	+	204	WP_006322256.1	hemolysin expression modulator Hha	Toxin
I6G64_RS06975	1456962..1457498	-	537	WP_006323971.1	cytochrome b/b6 domain-containing protein	-
I6G64_RS06980	1457559..1458980	-	1422	WP_062869867.1	N-acetylglucosamine-binding protein GbpA	-
I6G64_RS06985	1459260..1459634	+	375	WP_063198035.1	type II secretion system pilot lipoprotein GspS	-
I6G64_RS06990	1459631..1460461	-	831	WP_073972022.1	A24 family peptidase	-
I6G64_RS06995	1460494..1460955	-	462	WP_063198037.1	type II secretion system protein M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1455613..1472417	16804

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8085.45 Da
• Isoelectric Point: 6.9764

>T183158 WP_006322256.1 NZ_CP065673:1456518-1456721 [Serratia plymuthica]
MTKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 204 bp

>T183158 NZ_CP086725:56280-56762 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14203.92 Da
• Isoelectric Point: 4.5140

>AT183158 WP_004949348.1 NZ_CP065673:1456099-1456467 [Serratia plymuthica]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEGICTTMKT

Nucleotide

• Length: 369 bp

>AT183158 NZ_CP086725:56026-56292 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A1B1KM53

Antitoxin

• AlphaFold DB: S4YKH5