Detailed information of TA system TA183147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 48447..49120
• Replicon: plasmid unnamed1
• Accession: NZ_CP065669
• Organism: Sphingomonas paucimobilis strain FDAARGOS_908

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6G65_RS00235
• Protein ID: WP_042469253.1
• Coordinates: 48447..48860 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0C9NIS3
• Locus tag: I6G65_RS00240
• Protein ID: WP_042469255.1
• Coordinates: 48857..49120 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G65_RS00215	43946..44923	-	978	WP_030541261.1	DUF2493 domain-containing protein	-
I6G65_RS00220	45405..46517	+	1113	WP_042469251.1	hypothetical protein	-
I6G65_RS00225	46567..46887	-	321	WP_042469252.1	single-stranded DNA-binding protein	-
I6G65_RS00230	47148..48350	-	1203	WP_082052518.1	SEC-C domain-containing protein	-
I6G65_RS00235	48447..48860	-	414	WP_042469253.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6G65_RS00240	48857..49120	-	264	WP_042469255.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6G65_RS00245	49309..49812	-	504	WP_030541254.1	DUF736 domain-containing protein	-
I6G65_RS00250	49976..50539	-	564	WP_042469310.1	MucR family transcriptional regulator	-
I6G65_RS00255	50716..51033	-	318	WP_042469311.1	hypothetical protein	-
I6G65_RS00260	51344..51814	+	471	WP_156146122.1	hypothetical protein	-
I6G65_RS00265	51780..52277	-	498	WP_042469256.1	hypothetical protein	-
I6G65_RS00270	52418..52747	-	330	WP_042469258.1	hypothetical protein	-
I6G65_RS00275	52744..52938	-	195	WP_042469259.1	hypothetical protein	-
I6G65_RS00280	53072..53404	-	333	WP_042469261.1	DUF3768 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..352740	352740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (16-53)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14397.54 Da
• Isoelectric Point: 5.6141

>T183147 WP_042469253.1 NZ_CP065669:c48860-48447 [Sphingomonas paucimobilis]
VTQLYMLDTNIVSELARNPHGAVATRIAQVGADAICVSIITAAELRYGCAKKGSPRLLAQIEAILGSVSVLALDVPADTE
YGGIRAELESAGKPIGPNDLFIAAHAYALGATLVTANIGEFSRVRALKVENWLSDVH

Nucleotide

• Length: 414 bp

>T183147 NZ_CP086724:729025-729510 [Klebsiella pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9794.17 Da
• Isoelectric Point: 4.7010

>AT183147 WP_042469255.1 NZ_CP065669:c49120-48857 [Sphingomonas paucimobilis]
VTPDHEATVTREAKLFRNNKSQAVRIPADFELPGDRVMIHREGDRLVIEPIRRRNIVEVLASLEPLGPEDEFPDIDGTLL
PAKAVEL

Nucleotide

• Length: 264 bp

>AT183147 NZ_CP086724:728735-729028 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0C9NIS3