Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1029762..1030333 Replicon chromosome
Accession NZ_CP065662
Organism Aerococcus urinae strain FDAARGOS_911

Toxin (Protein)


Gene name mazF Uniprot ID A0A0X8FET5
Locus tag I6G68_RS04695 Protein ID WP_060778411.1
Coordinates 1029762..1030094 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A120I9U0
Locus tag I6G68_RS04700 Protein ID WP_060778410.1
Coordinates 1030088..1030333 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G68_RS04675 1025028..1025960 + 933 WP_060778415.1 glycosyltransferase family 2 protein -
I6G68_RS04680 1025969..1026880 + 912 WP_060778414.1 glycosyltransferase -
I6G68_RS04685 1026950..1028365 + 1416 WP_174519269.1 lipopolysaccharide biosynthesis protein -
I6G68_RS04690 1028485..1029651 + 1167 WP_060785032.1 nucleotide sugar dehydrogenase -
I6G68_RS04695 1029762..1030094 - 333 WP_060778411.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I6G68_RS04700 1030088..1030333 - 246 WP_060778410.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I6G68_RS04705 1031154..1031297 + 144 WP_156417358.1 hypothetical protein -
I6G68_RS04710 1031413..1031904 + 492 WP_060785031.1 hypothetical protein -
I6G68_RS04715 1032331..1032990 + 660 WP_144435191.1 helix-turn-helix domain-containing protein -
I6G68_RS04720 1032986..1033879 + 894 WP_144435190.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - ugd 1023922..1042820 18898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12735.81 Da        Isoelectric Point: 9.2408

>T183133 WP_060778411.1 NZ_CP065662:c1030094-1029762 [Aerococcus urinae]
MVKVKQGSIIKINLDPKQGHEQKGYRPYICLSHGIVTKYSNIAIFAPISNTKRKYPFYVELEQTKTTGKVLLDQLVTIDF
NARDYNYIEQVTDDILIDLLARVKVLFEKE

Download         Length: 333 bp

>T183133 NZ_CP086678:3998773-3999027 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9325.69 Da        Isoelectric Point: 4.7863

>AT183133 WP_060778410.1 NZ_CP065662:c1030333-1030088 [Aerococcus urinae]
MPLTINKWGNSSAIRLPKQLLEELNLSQGDKLDYQVKDGQLIIKKITEVPELTVKDLFENYDGAPVNEKPVLFEQRGNEK
W

Download         Length: 246 bp

>AT183133 NZ_CP086678:3998525-3998776 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X8FET5

Antitoxin

Source ID Structure
AlphaFold DB A0A120I9U0

References