Detailed information of TA system TA183131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-MazE
• Location: 122126..122715
• Replicon: chromosome
• Accession: NZ_CP065662
• Organism: Aerococcus urinae strain FDAARGOS_911

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A0X8FD65
• Locus tag: I6G68_RS00575
• Protein ID: WP_060777568.1
• Coordinates: 122126..122479 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0X8FCZ2
• Locus tag: I6G68_RS00580
• Protein ID: WP_060777567.1
• Coordinates: 122473..122715 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G68_RS00550	117181..118575	-	1395	WP_060777573.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
I6G68_RS00555	118872..120023	-	1152	WP_060777572.1	hypothetical protein	-
I6G68_RS00560	120036..120842	-	807	WP_060777571.1	hypothetical protein	-
I6G68_RS00565	120839..121528	-	690	WP_060777570.1	ABC transporter ATP-binding protein	-
I6G68_RS00570	121521..121898	-	378	WP_060777569.1	GntR family transcriptional regulator	-
I6G68_RS00575	122126..122479	-	354	WP_060777568.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6G68_RS00580	122473..122715	-	243	WP_060777567.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6G68_RS00585	122981..123538	+	558	WP_060777566.1	isochorismatase family protein	-
I6G68_RS00590	123664..124011	-	348	WP_060777565.1	hypothetical protein	-
I6G68_RS00595	124052..124276	-	225	WP_174519266.1	hypothetical protein	-
I6G68_RS00600	124450..125586	-	1137	WP_060777564.1	hypothetical protein	-
I6G68_RS00605	125719..126216	-	498	WP_195851504.1	hypothetical protein	-
I6G68_RS00610	126251..126865	-	615	WP_060777562.1	hypothetical protein	-
I6G68_RS00615	126913..127458	-	546	WP_060777561.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-99)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13876.81 Da
• Isoelectric Point: 8.0664

>T183131 WP_060777568.1 NZ_CP065662:c122479-122126 [Aerococcus urinae]
MVMRQGDIFYVNFNPNIGHEQRNSRPAIVLSHDLIFQTSRMAIVAPISTTKRNYPAYYELQETDHTKGKVLLDQTKSLDL
YHCQVSQENFIERAKPNEFKRIIHRYKLLFDLVDDYQ

Nucleotide

• Length: 354 bp

>T183131 NZ_CP086678:3222850-3223161 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9213.44 Da
• Isoelectric Point: 4.1954

>AT183131 WP_060777567.1 NZ_CP065662:c122715-122473 [Aerococcus urinae]
MQTKIAQWGNSKAIRIPSTIVKQLNLKDNQVLSLEIKDQALVIKPKEAETIQELFADYNTSFTYEEEMDWGPAVGEEIEW

Nucleotide

• Length: 243 bp

>AT183131 NZ_CP086678:3223158-3223577 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0X8FD65

Antitoxin

• AlphaFold DB: A0A0X8FCZ2