Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3425162..3425804 Replicon chromosome
Accession NZ_CP065650
Organism Bacillus cereus strain FDAARGOS_918

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag I6G75_RS18555 Protein ID WP_000635963.1
Coordinates 3425454..3425804 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag I6G75_RS18550 Protein ID WP_000004570.1
Coordinates 3425162..3425449 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G75_RS18525 3420478..3421440 + 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -
I6G75_RS18530 3421433..3422005 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
I6G75_RS18535 3422098..3422457 + 360 WP_000635040.1 holo-ACP synthase -
I6G75_RS18540 3422614..3423564 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
I6G75_RS18545 3423683..3424852 + 1170 WP_000390600.1 alanine racemase -
I6G75_RS18550 3425162..3425449 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
I6G75_RS18555 3425454..3425804 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I6G75_RS18560 3425872..3428040 + 2169 WP_000426222.1 RNA-binding transcriptional accessory protein -
I6G75_RS18565 3428098..3428214 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
I6G75_RS18570 3428410..3428868 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T183122 WP_000635963.1 NZ_CP065650:3425454-3425804 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T183122 NZ_CP086678:c1336260-1335973 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT183122 WP_000004570.1 NZ_CP065650:3425162-3425449 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT183122 NZ_CP086678:c1335986-1335684 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References