Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 297548..298523 Replicon chromosome
Accession NZ_CP065650
Organism Bacillus cereus strain FDAARGOS_918

Toxin (Protein)


Gene name spoIISA Uniprot ID C2NHW3
Locus tag I6G75_RS02315 Protein ID WP_002002796.1
Coordinates 297548..298285 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag I6G75_RS02320 Protein ID WP_000588712.1
Coordinates 298398..298523 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G75_RS02290 293040..293741 + 702 WP_001294994.1 hypothetical protein -
I6G75_RS02295 293869..294753 + 885 WP_000795511.1 hypothetical protein -
I6G75_RS02300 295061..295865 - 805 Protein_293 alpha-keto acid decarboxylase family protein -
I6G75_RS02305 295972..296454 + 483 WP_000191909.1 MarR family winged helix-turn-helix transcriptional regulator -
I6G75_RS02310 296622..297359 + 738 WP_000594157.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
I6G75_RS02315 297548..298285 + 738 WP_002002796.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
I6G75_RS02320 298398..298523 + 126 WP_000588712.1 hypothetical protein Antitoxin
I6G75_RS02325 298812..299093 - 282 WP_001041554.1 hypothetical protein -
I6G75_RS02330 299373..299609 - 237 WP_001021690.1 hypothetical protein -
I6G75_RS02335 299948..300124 + 177 WP_000852618.1 hypothetical protein -
I6G75_RS02340 300268..301737 + 1470 WP_000287550.1 beta-Ala-His dipeptidase -
I6G75_RS02345 302323..302793 - 471 WP_000771846.1 DUF5065 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28283.98 Da        Isoelectric Point: 8.2998

>T183121 WP_002002796.1 NZ_CP065650:297548-298285 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNAAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTTFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T183121 NZ_CP086678:c998127-997729 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT183121 WP_000588712.1 NZ_CP065650:298398-298523 [Bacillus cereus]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT183121 NZ_CP086678:c998423-998130 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D3YFM0

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References