Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3524582..3525200 Replicon chromosome
Accession NZ_CP065624
Organism Escherichia coli strain FDAARGOS_941

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6G98_RS18405 Protein ID WP_001291435.1
Coordinates 3524582..3524800 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6G98_RS18410 Protein ID WP_000344800.1
Coordinates 3524826..3525200 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G98_RS18370 3519697..3520008 - 312 WP_000409911.1 MGMT family protein -
I6G98_RS18380 3520387..3520740 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6G98_RS18385 3520782..3522332 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6G98_RS18390 3522496..3522966 - 471 WP_000136192.1 YlaC family protein -
I6G98_RS18395 3523082..3523597 - 516 WP_197932987.1 maltose O-acetyltransferase -
I6G98_RS18400 3523647..3524344 + 698 Protein_3430 IS1-like element IS1A family transposase -
I6G98_RS18405 3524582..3524800 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6G98_RS18410 3524826..3525200 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6G98_RS18415 3525746..3528895 - 3150 WP_197932774.1 multidrug efflux RND transporter permease subunit AcrB -
I6G98_RS18420 3528918..3530111 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3523841..3524344 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T183075 WP_001291435.1 NZ_CP065624:c3524800-3524582 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T183075 NZ_CP086659:841459-841815 [Staphylococcus capitis]
ATGATTAGAAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTTGT
AATTATACAAAATGATACTGGTAATAAATATAGTCCAACTGTCATAGTTGCTGCTATTACAGGTAGGATTAATAAAGCGA
AGATACCAACCCATGTAGAAATTGAAAAGAAAAAATATAAGTTAGATAAAGACTCTGTTATCTTACTTGAACAAATTAGA
ACACTAGATAAAAAACGTTTAAAAGAGAAGTTGACCTATTTGTCTGAAAGTAAGATGAAGGAAGTTGACAACGCCCTAGA
TATCAGTCTAGGATTACATAATTTTGACCATCAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT183075 WP_000344800.1 NZ_CP065624:c3525200-3524826 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT183075 NZ_CP086659:841292-841462 [Staphylococcus capitis]
ATGTTATCTTTTAATCAATCTAGGAGTCACAGTCTTGAACAATCATTAAAAGAAGGCTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACGGAAGCTTTTCCCATCGAATGTGAAGCTTGCGATTGTAATGAAACATATCTTACTTCTAACTCTA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References