Detailed information of TA system TA183074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3488202..3489039
• Replicon: chromosome
• Accession: NZ_CP065624
• Organism: Escherichia coli strain FDAARGOS_941

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: I6G98_RS18220
• Protein ID: WP_000227784.1
• Coordinates: 3488202..3488744 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: I6G98_RS18225
• Protein ID: WP_032359196.1
• Coordinates: 3488728..3489039 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G98_RS18200	3483741..3484652	-	912	WP_032359198.1	2-dehydropantoate 2-reductase	-
I6G98_RS18205	3484820..3485311	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
I6G98_RS18210	3485439..3486803	-	1365	WP_032314626.1	MFS transporter	-
I6G98_RS18215	3487211..3488146	+	936	WP_032359197.1	sel1 repeat family protein	-
I6G98_RS18220	3488202..3488744	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
I6G98_RS18225	3488728..3489039	-	312	WP_032359196.1	DUF1778 domain-containing protein	Antitoxin
I6G98_RS18230	3489224..3490114	-	891	WP_000971336.1	heme o synthase	-
I6G98_RS18235	3490126..3490455	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
I6G98_RS18240	3490455..3491069	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
I6G98_RS18245	3491059..3493050	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
I6G98_RS18250	3493072..3494019	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T183074 WP_000227784.1 NZ_CP065624:c3488744-3488202 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T183074 NZ_CP086659:830564-830770 [Staphylococcus capitis]
ATGGAAAATGGAATTGTTTATGTTGCTAATATTGAGACTATAGAGGAAGAAAACCAATTAAAGACACGTTTAGAAAATAT
GATTGGTGTCGAAAAAGTAGATATTGATAGTAAAGTAGGAGAAATAAAGGTATTATTTGAAACGCCTGCGAATTTAAATA
GTATTGAAAAAGAAGTATATGATTCTGGCTTTCGCGTGCGTTATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11338.99 Da
• Isoelectric Point: 10.2456

>AT183074 WP_032359196.1 NZ_CP065624:c3489039-3488728 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRHRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT183074 NZ_CP086659:830256-830549 [Staphylococcus capitis]
GTGACTGAAAATGAAACTGCACATCACTCAGAGCAAACGAAAGCAAATTTAAAGTCACGATTAAATCGTATCGAAGGTCA
AGTACGTGCAATTAATCGAATGATTGAAGACGATGTGTATTGCGATGATGTTTTAACTCAAATAAGAGCGACACGCTCTG
CTTTAAACAGTGTGGCTACTAAATTGTTAGATCATCATATGAAAAGTTGTATTATGAATAAAGTGAATAATGGTGCACAA
GAAGAAGCGATGGAAGAACTATTGGTTACTTTTCAAAAATTAATTAAAGATTAA

Structures

Toxin

• PDB: 7BYY