Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1930875..1931506 Replicon chromosome
Accession NZ_CP065624
Organism Escherichia coli strain FDAARGOS_941

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag I6G98_RS10670 Protein ID WP_001259385.1
Coordinates 1931231..1931506 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag I6G98_RS10665 Protein ID WP_000593555.1
Coordinates 1930875..1931234 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G98_RS10640 1927007..1927951 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
I6G98_RS10645 1927948..1928781 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
I6G98_RS10650 1928781..1929545 + 765 WP_001136223.1 nickel import ATP-binding protein NikD -
I6G98_RS10655 1929542..1930348 + 807 WP_000173703.1 nickel import ATP-binding protein NikE -
I6G98_RS10660 1930354..1930755 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
I6G98_RS10665 1930875..1931234 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6G98_RS10670 1931231..1931506 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
I6G98_RS10675 1931579..1932703 - 1125 WP_001216257.1 ABC transporter permease -
I6G98_RS10680 1932703..1935438 - 2736 WP_032359128.1 ribosome-associated ATPase/putative transporter RbbA -
I6G98_RS10685 1935435..1936502 - 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T183069 WP_001259385.1 NZ_CP065624:c1931506-1931231 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T183069 NZ_CP086627:c4996546-4996235 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTCACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT183069 WP_000593555.1 NZ_CP065624:c1931234-1930875 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT183069 NZ_CP086627:c4996234-4995944 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References