Detailed information of TA system TA183063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 880191..880816
• Replicon: chromosome
• Accession: NZ_CP065624
• Organism: Escherichia coli strain FDAARGOS_941

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6G98_RS05345
• Protein ID: WP_000911330.1
• Coordinates: 880191..880589 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: I6G98_RS05350
• Protein ID: WP_000450524.1
• Coordinates: 880589..880816 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G98_RS05325	876069..876269	+	201	WP_000383836.1	YpfN family protein	-
I6G98_RS05330	876379..877077	-	699	WP_000679812.1	esterase	-
I6G98_RS05335	877151..879166	-	2016	WP_032359650.1	tRNA cytosine(34) acetyltransferase TmcA	-
I6G98_RS05340	879181..880044	-	864	WP_001267495.1	neutral zinc metallopeptidase	-
I6G98_RS05345	880191..880589	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I6G98_RS05350	880589..880816	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I6G98_RS05355	880970..881683	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
I6G98_RS05360	881896..882930	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
I6G98_RS05365	882947..883825	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
I6G98_RS05370	883971..884543	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
I6G98_RS05375	884543..885013	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T183063 WP_000911330.1 NZ_CP065624:c880589-880191 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T183063 NZ_CP086627:3893267-3893485 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT183063 WP_000450524.1 NZ_CP065624:c880816-880589 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT183063 NZ_CP086627:3892867-3893241 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33