Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 45577..46334 Replicon plasmid unnamed
Accession NZ_CP065623
Organism Escherichia coli strain FDAARGOS_941

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag I6G98_RS00325 Protein ID WP_000501969.1
Coordinates 45849..46334 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag I6G98_RS00320 Protein ID WP_011114751.1
Coordinates 45577..45861 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G98_RS00300 41734..42531 + 798 WP_032358891.1 IS21-like element IS21 family helper ATPase IstB -
I6G98_RS00305 42591..42854 + 264 Protein_60 transposase -
I6G98_RS00310 42909..44122 + 1214 WP_187415725.1 IS3 family transposase -
I6G98_RS00315 44511..44675 - 165 WP_001346193.1 hypothetical protein -
I6G98_RS00320 45577..45861 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
I6G98_RS00325 45849..46334 + 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
I6G98_RS00330 46528..46842 + 315 Protein_65 IS3 family transposase -
I6G98_RS00335 46917..49404 - 2488 Protein_66 Tn3 family transposase -
I6G98_RS00340 49576..50745 - 1170 WP_197932730.1 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospI / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / senB 1..123793 123793
- inside IScluster/Tn - ospG / ipaH9.8 / ospI / ospD3/senA / ospC1 16565..58071 41506


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T183052 WP_000501969.1 NZ_CP065623:45849-46334 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T183052 NZ_CP086627:c989688-989314 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT183052 WP_011114751.1 NZ_CP065623:45577-45861 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT183052 NZ_CP086627:c990109-989735 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References