Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 28875..29434 | Replicon | plasmid unnamed |
| Accession | NZ_CP065623 | ||
| Organism | Escherichia coli strain FDAARGOS_941 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | I6G98_RS00205 | Protein ID | WP_000421262.1 |
| Coordinates | 28875..29150 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q327A8 |
| Locus tag | I6G98_RS00210 | Protein ID | WP_001178088.1 |
| Coordinates | 29150..29434 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6G98_RS00170 | 25285..25380 | - | 96 | Protein_33 | transposase domain-containing protein | - |
| I6G98_RS00175 | 25416..25721 | - | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
| I6G98_RS00180 | 25723..25941 | - | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| I6G98_RS00185 | 26477..27568 | - | 1092 | Protein_36 | IS66 family transposase | - |
| I6G98_RS00190 | 27576..28145 | + | 570 | Protein_37 | plasmid segregation protein ParM | - |
| I6G98_RS00195 | 28148..28499 | + | 352 | Protein_38 | plasmid partitioning/stability family protein | - |
| I6G98_RS00200 | 28606..28743 | + | 138 | WP_000766699.1 | hypothetical protein | - |
| I6G98_RS00205 | 28875..29150 | - | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| I6G98_RS00210 | 29150..29434 | - | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| I6G98_RS00215 | 29958..30137 | + | 180 | Protein_42 | MFS transporter | - |
| I6G98_RS00220 | 30187..30396 | + | 210 | Protein_43 | peptidoglycan-binding protein | - |
| I6G98_RS00225 | 30496..30681 | - | 186 | Protein_44 | ATP-binding protein | - |
| I6G98_RS00230 | 31017..31487 | + | 471 | Protein_45 | IS1 family transposase | - |
| I6G98_RS00235 | 31646..32284 | + | 639 | WP_000607008.1 | type III secretion system effector OspI | - |
| I6G98_RS00240 | 32399..32668 | + | 270 | Protein_47 | transposase | - |
| I6G98_RS00245 | 32756..33984 | + | 1229 | WP_148717946.1 | IS3-like element IS2 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospG / ipaH9.8 / ospI / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / senB | 1..123793 | 123793 | |
| - | inside | IScluster/Tn | - | ospG / ipaH9.8 / ospI / ospD3/senA / ospC1 | 16565..58071 | 41506 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T183051 WP_000421262.1 NZ_CP065623:c29150-28875 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T183051 NZ_CP086627:c914999-914622 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10497.93 Da Isoelectric Point: 6.2150
>AT183051 WP_001178088.1 NZ_CP065623:c29434-29150 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT183051 NZ_CP086627:c915457-915089 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q327A8 |