Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 25416..25941 Replicon plasmid unnamed
Accession NZ_CP065623
Organism Escherichia coli strain FDAARGOS_941

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag I6G98_RS00175 Protein ID WP_001159860.1
Coordinates 25416..25721 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag I6G98_RS00180 Protein ID WP_000813626.1
Coordinates 25723..25941 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G98_RS00140 21178..21381 - 204 Protein_27 transposase domain-containing protein -
I6G98_RS00145 21444..21728 - 285 Protein_28 IS66 family insertion sequence element accessory protein TnpB -
I6G98_RS00150 22067..22657 - 591 WP_000705601.1 type III secretion system effector protein kinase OspG -
I6G98_RS00155 22686..22912 - 227 Protein_30 type II toxin-antitoxin system RelE/ParE family toxin -
I6G98_RS00160 22909..23178 - 270 WP_000079956.1 hypothetical protein -
I6G98_RS00165 23386..25023 - 1638 WP_000936801.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
I6G98_RS00170 25285..25380 - 96 Protein_33 transposase domain-containing protein -
I6G98_RS00175 25416..25721 - 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
I6G98_RS00180 25723..25941 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6G98_RS00185 26477..27568 - 1092 Protein_36 IS66 family transposase -
I6G98_RS00190 27576..28145 + 570 Protein_37 plasmid segregation protein ParM -
I6G98_RS00195 28148..28499 + 352 Protein_38 plasmid partitioning/stability family protein -
I6G98_RS00200 28606..28743 + 138 WP_000766699.1 hypothetical protein -
I6G98_RS00205 28875..29150 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
I6G98_RS00210 29150..29434 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein -
I6G98_RS00215 29958..30137 + 180 Protein_42 MFS transporter -
I6G98_RS00220 30187..30396 + 210 Protein_43 peptidoglycan-binding protein -
I6G98_RS00225 30496..30681 - 186 Protein_44 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospI / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / senB 1..123793 123793
- inside IScluster/Tn - ospG / ipaH9.8 / ospI / ospD3/senA / ospC1 16565..58071 41506


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T183050 WP_001159860.1 NZ_CP065623:c25721-25416 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T183050 NZ_CP086627:c707978-707514 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT183050 WP_000813626.1 NZ_CP065623:c25941-25723 [Escherichia coli]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT183050 NZ_CP086627:c708313-707978 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References