Detailed information of TA system TA183049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 136251..136776
• Replicon: plasmid unnamed1
• Accession: NZ_CP065616
• Organism: Escherichia coli strain FDAARGOS_943

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: I6H00_RS27195
• Protein ID: WP_197940177.1
• Coordinates: 136471..136776 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q7BEK0
• Locus tag: I6H00_RS27190
• Protein ID: WP_000813626.1
• Coordinates: 136251..136469 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H00_RS27145	131281..131460	-	180	Protein_147	MFS transporter	-
I6H00_RS27150	131984..132268	+	285	WP_001178088.1	ribbon-helix-helix domain-containing protein	-
I6H00_RS27155	132268..132543	+	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6H00_RS27160	132675..132812	-	138	WP_000766699.1	hypothetical protein	-
I6H00_RS27165	132919..133269	-	351	WP_197940176.1	plasmid partitioning/stability family protein	-
I6H00_RS27170	133272..133696	-	425	Protein_152	plasmid segregation protein ParM	-
I6H00_RS27175	133751..134448	+	698	Protein_153	IS1 family transposase	-
I6H00_RS27180	134461..134616	-	156	Protein_154	recombinase	-
I6H00_RS27185	134624..135715	+	1092	Protein_155	IS66 family transposase	-
I6H00_RS27190	136251..136469	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6H00_RS27195	136471..136776	+	306	WP_197940177.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6H00_RS27200	136812..136907	+	96	Protein_158	transposase domain-containing protein	-
I6H00_RS27205	137169..138806	+	1638	WP_000936806.1	T3SS effector E3 ubiquitin-protein ligase IpaH9.8	-
I6H00_RS27210	139014..139283	+	270	WP_000079957.1	hypothetical protein	-
I6H00_RS27215	139280..139506	+	227	Protein_161	type II toxin-antitoxin system RelE/ParE family toxin	-
I6H00_RS27220	139535..140125	+	591	WP_000705601.1	type III secretion system effector protein kinase OspG	-
I6H00_RS27225	140268..140965	+	698	Protein_163	IS1 family transposase	-
I6H00_RS27230	141523..141675	+	153	WP_197940178.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeI / faeH / faeF / faeE / faeD / faeC / bfpA / bfpD / bfpE / bfpF / ipaH9.8 / ospG / ospC2 / icsP/sopA / ospC3	1..155369	155369
	-	inside	IScluster/Tn	-	ipaH9.8 / ospG / ospC2 / icsP/sopA / ospC3	121332..155306	33974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11702.57 Da
• Isoelectric Point: 6.4674

>T183049 WP_197940177.1 NZ_CP065616:136471-136776 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMLWGI

Nucleotide

• Length: 306 bp

>T183049 NZ_CP086627:260341-260448 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8333.36 Da
• Isoelectric Point: 6.2838

>AT183049 WP_000813626.1 NZ_CP065616:136251-136469 [Escherichia coli]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT183049 NZ_CP086627:c260292-260229 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Antitoxin

• AlphaFold DB: Q7BEK0