Detailed information of TA system TA183048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 131984..132543
• Replicon: plasmid unnamed1
• Accession: NZ_CP065616
• Organism: Escherichia coli strain FDAARGOS_943

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2AJT2
• Locus tag: I6H00_RS27155
• Protein ID: WP_000421262.1
• Coordinates: 132268..132543 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q327A8
• Locus tag: I6H00_RS27150
• Protein ID: WP_001178088.1
• Coordinates: 131984..132268 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H00_RS27115	127358..127642	-	285	Protein_141	transposase	-
I6H00_RS27120	127693..128538	+	846	Protein_142	IS66-like element ISSfl3 family transposase	-
I6H00_RS27125	128648..129876	+	1229	WP_171764973.1	IS3-like element IS2 family transposase	-
I6H00_RS27130	129931..130401	-	471	Protein_144	IS1 family transposase	-
I6H00_RS27135	130737..130922	+	186	Protein_145	ATP-binding protein	-
I6H00_RS27140	131022..131231	-	210	Protein_146	peptidoglycan-binding protein	-
I6H00_RS27145	131281..131460	-	180	Protein_147	MFS transporter	-
I6H00_RS27150	131984..132268	+	285	WP_001178088.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6H00_RS27155	132268..132543	+	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H00_RS27160	132675..132812	-	138	WP_000766699.1	hypothetical protein	-
I6H00_RS27165	132919..133269	-	351	WP_197940176.1	plasmid partitioning/stability family protein	-
I6H00_RS27170	133272..133696	-	425	Protein_152	plasmid segregation protein ParM	-
I6H00_RS27175	133751..134448	+	698	Protein_153	IS1 family transposase	-
I6H00_RS27180	134461..134616	-	156	Protein_154	recombinase	-
I6H00_RS27185	134624..135715	+	1092	Protein_155	IS66 family transposase	-
I6H00_RS27190	136251..136469	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	-
I6H00_RS27195	136471..136776	+	306	WP_197940177.1	type II toxin-antitoxin system toxin CcdB	-
I6H00_RS27200	136812..136907	+	96	Protein_158	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeI / faeH / faeF / faeE / faeD / faeC / bfpA / bfpD / bfpE / bfpF / ipaH9.8 / ospG / ospC2 / icsP/sopA / ospC3	1..155369	155369
	-	inside	IScluster/Tn	-	ipaH9.8 / ospG / ospC2 / icsP/sopA / ospC3	121332..155306	33974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10766.48 Da
• Isoelectric Point: 9.9324

>T183048 WP_000421262.1 NZ_CP065616:132268-132543 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T183048 NZ_CP086627:259858-259965 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10497.93 Da
• Isoelectric Point: 6.2150

>AT183048 WP_001178088.1 NZ_CP065616:131984-132268 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Nucleotide

• Length: 285 bp

>AT183048 NZ_CP086627:c259809-259746 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Toxin

• AlphaFold DB: A0A822PVM3

Antitoxin

• AlphaFold DB: Q327A8