Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56910..57435 Replicon plasmid unnamed1
Accession NZ_CP065616
Organism Escherichia coli strain FDAARGOS_943

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I6H00_RS26735 Protein ID WP_001159871.1
Coordinates 56910..57215 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6H00_RS26740 Protein ID WP_000813634.1
Coordinates 57217..57435 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H00_RS26700 52185..52577 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
I6H00_RS26705 52582..53145 - 564 Protein_59 plasmid segregation protein parM -
I6H00_RS26710 53210..54366 + 1157 WP_197933473.1 IS3 family transposase -
I6H00_RS26715 54410..54820 - 411 Protein_61 plasmid segregation protein parM -
I6H00_RS26720 55049..55693 + 645 WP_000633911.1 AAA family ATPase -
I6H00_RS26725 55687..55962 + 276 WP_000239529.1 hypothetical protein -
I6H00_RS26730 56100..56909 - 810 WP_000016979.1 site-specific integrase -
I6H00_RS26735 56910..57215 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I6H00_RS26740 57217..57435 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6H00_RS26745 58183..59339 + 1157 WP_197939898.1 IS3 family transposase -
I6H00_RS26750 59384..61402 + 2019 Protein_68 acid-sensing system histidine kinase EvgS -
I6H00_RS26755 61458..61613 - 156 Protein_69 CoA:oxalate CoA-transferase -
I6H00_RS26760 61669..62087 + 419 Protein_70 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - faeI / faeH / faeF / faeE / faeD / faeC / bfpA / bfpD / bfpE / bfpF / ipaH9.8 / ospG / ospC2 / icsP/sopA / ospC3 1..155369 155369
- inside IScluster/Tn - - 43603..76954 33351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T183047 WP_001159871.1 NZ_CP065616:c57215-56910 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T183047 NZ_CP086627:259374-259481 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT183047 WP_000813634.1 NZ_CP065616:c57435-57217 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT183047 NZ_CP086627:c259316-259262 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References