Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3536..4299 Replicon plasmid unnamed1
Accession NZ_CP065616
Organism Escherichia coli strain FDAARGOS_943

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag I6H00_RS26440 Protein ID WP_000405248.1
Coordinates 3536..4018 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A0M1UJ31
Locus tag I6H00_RS26445 Protein ID WP_000100678.1
Coordinates 4009..4299 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H00_RS26415 482..838 + 357 Protein_1 transposase -
I6H00_RS26420 872..1093 + 222 Protein_2 IS4 family transposase -
I6H00_RS26425 1148..1897 - 750 WP_000828661.1 phosphatase PAP2 family protein -
I6H00_RS26430 2165..2276 - 112 Protein_4 IS1 family transposase -
I6H00_RS26440 3536..4018 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
I6H00_RS26445 4009..4299 - 291 WP_000100678.1 DUF1778 domain-containing protein Antitoxin
I6H00_RS26455 6223..8334 + 2112 WP_197940183.1 E3 ubiquitin--protein ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - faeI / faeH / faeF / faeE / faeD / faeC / bfpA / bfpD / bfpE / bfpF / ipaH9.8 / ospG / ospC2 / icsP/sopA / ospC3 1..155369 155369
- inside IScluster/Tn - - 34..17327 17293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(15-90)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T183046 WP_000405248.1 NZ_CP065616:c4018-3536 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T183046 NZ_CP086627:203858-204043 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 11182.66 Da        Isoelectric Point: 5.1974

>AT183046 WP_000100678.1 NZ_CP065616:c4299-4009 [Escherichia coli]
MSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQ
NAEGRERLMAVKPEWK

Download         Length: 291 bp

>AT183046 NZ_CP086627:204060-204470 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M1UJ31

References