Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4625453..4626107 Replicon chromosome
Accession NZ_CP065615
Organism Escherichia coli strain FDAARGOS_943

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I6H00_RS23285 Protein ID WP_000244777.1
Coordinates 4625700..4626107 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A377CRZ7
Locus tag I6H00_RS23280 Protein ID WP_038340210.1
Coordinates 4625453..4625719 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H00_RS23260 4621541..4622974 - 1434 WP_038340246.1 6-phospho-beta-glucosidase BglA -
I6H00_RS23265 4623019..4623330 + 312 WP_038340212.1 N(4)-acetylcytidine aminohydrolase -
I6H00_RS23270 4623494..4624153 + 660 WP_000250274.1 hemolysin III family protein -
I6H00_RS23275 4624230..4625210 - 981 WP_038340211.1 tRNA-modifying protein YgfZ -
I6H00_RS23280 4625453..4625719 + 267 WP_038340210.1 FAD assembly factor SdhE Antitoxin
I6H00_RS23285 4625700..4626107 + 408 WP_000244777.1 toxin CptA Toxin
I6H00_RS23290 4626147..4626668 - 522 WP_001055874.1 flavodoxin FldB -
I6H00_RS23295 4626780..4627676 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6H00_RS23300 4627701..4628411 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6H00_RS23305 4628417..4630150 + 1734 WP_038340206.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T183044 WP_000244777.1 NZ_CP065615:4625700-4626107 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T183044 NZ_CP086626:c5027959-5027648 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTCACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10577.15 Da        Isoelectric Point: 5.1599

>AT183044 WP_038340210.1 NZ_CP065615:4625453-4625719 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPTDAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT183044 NZ_CP086626:c5027647-5027357 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
AlphaFold DB A0A377CRZ7

References