Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3220810..3221307 Replicon chromosome
Accession NZ_CP065615
Organism Escherichia coli strain FDAARGOS_943

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6H00_RS16420 Protein ID WP_001173343.1
Coordinates 3220810..3220983 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6H00_RS16425 Protein ID WP_000398619.1
Coordinates 3221011..3221307 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H00_RS16405 3216562..3218019 + 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -
I6H00_RS16415 3219174..3220691 + 1518 WP_001295089.1 lysine--tRNA ligase -
I6H00_RS16420 3220810..3220983 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6H00_RS16425 3221011..3221307 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6H00_RS16430 3221534..3221806 - 273 WP_197939670.1 N-acetyltransferase -
I6H00_RS16435 3221818..3222048 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
I6H00_RS16440 3222229..3223860 + 1632 WP_024243493.1 sensor histidine kinase -
I6H00_RS16445 3223857..3224576 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3211521..3221307 9786
- flank IS/Tn - - 3218134..3218649 515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T183042 WP_001173343.1 NZ_CP065615:c3220983-3220810 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T183042 NZ_CP086626:c4634879-4634529 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT183042 WP_000398619.1 NZ_CP065615:c3221307-3221011 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT183042 NZ_CP086626:c4635124-4634873 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References